論文 - 詳細
| RRC ID | 46486 |
|---|---|
| 著者 | Hagiwara K, Nagamori S, Umemura YM, Ohgaki R, Tanaka H, Murata D, Nakagomi S, Nomura KH, Kage-Nakadai E, Mitani S, Nomura K, Kanai Y. |
| タイトル | NRFL-1, the C. elegans NHERF orthologue, interacts with amino acid transporter 6 (AAT-6) for age-dependent maintenance of AAT-6 on the membrane. |
| ジャーナル | PLoS One |
| Abstract |
The NHERF (Na(+)/H(+) exchanger regulatory factor) family has been proposed to play a key role in regulating transmembrane protein localization and retention at the plasma membrane. Due to the high homology between the family members, potential functional compensations have been a concern in sorting out the function of individual NHERF numbers. Here, we studied C. elegans NRFL-1 (C01F6.6) (nherf-like protein 1), the sole C. elegans orthologue of the NHERF family, which makes worm a model with low genetic redundancy of NHERF homologues. Integrating bioinformatic knowledge of C. elegans proteins into yeast two-hybrid scheme, we identified NRFL-1 as an interactor of AAT-6, a member of the C. elegans AAT (amino acid transporter) family. A combination of GST pull-down assay, localization study, and co-immunoprecipitation confirmed the binding and characterized the PDZ interaction. AAT-6 localizes to the luminal membrane even in the absence of NRFL-1 when the worm is up to four-day old. A fluorescence recovery after photobleaching (FRAP) analysis suggested that NRFL-1 immobilizes AAT-6 at the luminal membrane. When the nrfl-1 deficient worm is six-day or older, in contrast, the membranous localization of AAT-6 is not observed, whereas AAT-6 tightly localizes to the membrane in worms with NRFL-1. Sorting out the in vivo functions of the C. elegans NHERF protein, we found that NRFL-1, a PDZ-interactor of AAT-6, is responsible for the immobilization and the age-dependent maintenance of AAT-6 on the intestinal luminal membrane. |
| 巻・号 | 7(8) |
| ページ | e43050 |
| 公開日 | 2012-1-1 |
| DOI | 10.1371/journal.pone.0043050 |
| PII | PONE-D-11-16556 |
| PMID | 22916205 |
| PMC | PMC3419730 |
| MeSH | Amino Acid Transport Systems / genetics Amino Acid Transport Systems / metabolism* Animals Caenorhabditis elegans / metabolism* Caenorhabditis elegans Proteins / genetics Caenorhabditis elegans Proteins / metabolism* Intestinal Mucosa / metabolism Phosphoproteins / genetics Phosphoproteins / metabolism* Phosphorylation Protein Binding Sodium-Hydrogen Exchangers / genetics Sodium-Hydrogen Exchangers / metabolism* Two-Hybrid System Techniques |
| IF | 2.74 |
| 引用数 | 6 |
| WOS 分野 | BIOCHEMISTRY & MOLECULAR BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 3 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 線虫 | tm2881 tm3501 |