| RRC ID |
46486
|
| 著者 |
Hagiwara K, Nagamori S, Umemura YM, Ohgaki R, Tanaka H, Murata D, Nakagomi S, Nomura KH, Kage-Nakadai E, Mitani S, Nomura K, Kanai Y.
|
| タイトル |
NRFL-1, the C. elegans NHERF orthologue, interacts with amino acid transporter 6 (AAT-6) for age-dependent maintenance of AAT-6 on the membrane.
|
| ジャーナル |
PLoS One
|
| Abstract |
The NHERF (Na(+)/H(+) exchanger regulatory factor) family has been proposed to play a key role in regulating transmembrane protein localization and retention at the plasma membrane. Due to the high homology between the family members, potential functional compensations have been a concern in sorting out the function of individual NHERF numbers. Here, we studied C. elegans NRFL-1 (C01F6.6) (nherf-like protein 1), the sole C. elegans orthologue of the NHERF family, which makes worm a model with low genetic redundancy of NHERF homologues. Integrating bioinformatic knowledge of C. elegans proteins into yeast two-hybrid scheme, we identified NRFL-1 as an interactor of AAT-6, a member of the C. elegans AAT (amino acid transporter) family. A combination of GST pull-down assay, localization study, and co-immunoprecipitation confirmed the binding and characterized the PDZ interaction. AAT-6 localizes to the luminal membrane even in the absence of NRFL-1 when the worm is up to four-day old. A fluorescence recovery after photobleaching (FRAP) analysis suggested that NRFL-1 immobilizes AAT-6 at the luminal membrane. When the nrfl-1 deficient worm is six-day or older, in contrast, the membranous localization of AAT-6 is not observed, whereas AAT-6 tightly localizes to the membrane in worms with NRFL-1. Sorting out the in vivo functions of the C. elegans NHERF protein, we found that NRFL-1, a PDZ-interactor of AAT-6, is responsible for the immobilization and the age-dependent maintenance of AAT-6 on the intestinal luminal membrane.
|
| 巻・号 |
7(8)
|
| ページ |
e43050
|
| 公開日 |
2012-1-1
|
| DOI |
10.1371/journal.pone.0043050
|
| PII |
PONE-D-11-16556
|
| PMID |
22916205
|
| PMC |
PMC3419730
|
| MeSH |
Amino Acid Transport Systems / genetics
Amino Acid Transport Systems / metabolism*
Animals
Caenorhabditis elegans / metabolism*
Caenorhabditis elegans Proteins / genetics
Caenorhabditis elegans Proteins / metabolism*
Intestinal Mucosa / metabolism
Phosphoproteins / genetics
Phosphoproteins / metabolism*
Phosphorylation
Protein Binding
Sodium-Hydrogen Exchangers / genetics
Sodium-Hydrogen Exchangers / metabolism*
Two-Hybrid System Techniques
|
| IF |
2.74
|
| 引用数 |
6
|
|
WOS 分野
|
BIOCHEMISTRY & MOLECULAR BIOLOGY
|
| リソース情報 |
| 線虫 |
tm2881
tm3501 |
