RRC ID 46504
Author Bamunusinghe D, Naghashfar Z, Buckler-White A, Plishka R, Baliji S, Liu Q, Kassner J, Oler AJ, Hartley J, Kozak CA.
Title Sequence Diversity, Intersubgroup Relationships, and Origins of the Mouse Leukemia Gammaretroviruses of Laboratory and Wild Mice.
Journal J Virol
Abstract UNLABELLED:Mouse leukemia viruses (MLVs) are found in the common inbred strains of laboratory mice and in the house mouse subspecies ofMus musculus Receptor usage and envelope (env) sequence variation define three MLV host range subgroups in laboratory mice: ecotropic, polytropic, and xenotropic MLVs (E-, P-, and X-MLVs, respectively). These exogenous MLVs derive from endogenous retroviruses (ERVs) that were acquired by the wild mouse progenitors of laboratory mice about 1 million years ago. We analyzed the genomes of seven MLVs isolated from Eurasian and American wild mice and three previously sequenced MLVs to describe their relationships and identify their possible ERV progenitors. The phylogenetic tree based on the receptor-determining regions ofenvproduced expected host range clusters, but these clusters are not maintained in trees generated from other virus regions. Colinear alignments of the viral genomes identified segmental homologies to ERVs of different host range subgroups. Six MLVs show close relationships to a small xenotropic ERV subgroup largely confined to the inbred mouse Y chromosome.envvariations define three E-MLV subtypes, one of which carries duplications of various sizes, sequences, and locations in the proline-rich region ofenv Outside theenvregion, all E-MLVs are related to different nonecotropic MLVs. These results document the diversity in gammaretroviruses isolated from globally distributedMussubspecies, provide insight into their origins and relationships, and indicate that recombination has had an important role in the evolution of these mutagenic and pathogenic agents.
IMPORTANCE:Laboratory mice carry mouse leukemia viruses (MLVs) of three host range groups which were acquired from their wild mouse progenitors. We sequenced the complete genomes of seven infectious MLVs isolated from geographically separated Eurasian and American wild mice and compared them with endogenous germ line retroviruses (ERVs) acquired early in house mouse evolution. We did this because the laboratory mouse viruses derive directly from specific ERVs or arise by recombination between different ERVs. The six distinctively different wild mouse viruses appear to be recombinants, often involving different host range subgroups, and most are related to a distinctive, largely Y-chromosome-linked MLV ERV subtype. MLVs with ecotropic host ranges show the greatest variability with extensive inter- and intrasubtype envelope differences and with homologies to other host range subgroups outside the envelope. The sequence diversity among these wild mouse isolates helps define their relationships and origins and emphasizes the importance of recombination in their evolution.
Volume 90(8)
Pages 4186-98
Published 2016-4-1
DOI 10.1128/JVI.03186-15
PII JVI.03186-15
PMID 26865715
PMC PMC4810534
MeSH Animals Animals, Laboratory / virology Animals, Wild / virology Base Sequence Genes, pol Genetic Variation* Genome, Viral Leukemia Virus, Murine / classification Leukemia Virus, Murine / genetics* Mice / genetics Mice / virology* Mice, Inbred Strains Molecular Sequence Data RNA, Viral Sequence Analysis, RNA
IF 4.501
Times Cited 7
DNA material Genomic DNA of mouse: Mus musculus musculus PWK RBRC00213 male (RDB06980) Mus musculus molossinus STM1 RBRC00265 male (RDB06981) Mus musculus molossinus STM2 RBRC00266 male (RDB06982) Mus musculus molossinus Mol-KOR5/Stm RBRC00428 male (RDB06983) Mus musculus molossinus Mol-KOR7/Stm RBRC00429 male (RDB06984) Mus musculus molossinus Mol-AIZ/Stm RBRC00430 male (RDB06985) Mus musculus molossinus Mol-Mae/Stm RBRC00431 male (RDB06986) Mus musculus musculus BLG2/Ms RBRC00653 male (RDB06987) Mus musculus spp. KJR/Ms RBRC00655 male (RDB06988) Mus musculus musculus MBT/Pas RBRC01164 male (RDB06992) Mus musculus castaneus MYS/Mz RBRC01196 male (RDB06993) Mus musculus musculus Akt/TUA RBRC01238 male (RDB06994) Mus musculus musculus Ast/TUA RBRC01249 male (RDB06995) Mus musculus molossinus MOM RBRC01837 male (RDB06996)