論文 - 詳細
| RRC ID | 46523 |
|---|---|
| 著者 | Tulgren ED, Baker ST, Rapp L, Gurney AM, Grill B. |
| タイトル | PPM-1, a PP2Cα/β phosphatase, regulates axon termination and synapse formation in Caenorhabditis elegans. |
| ジャーナル | Genetics |
| Abstract |
The PHR (Pam/Highwire/RPM-1) proteins are evolutionarily conserved ubiquitin ligases that regulate axon guidance and synapse formation in Caenorhabditis elegans, Drosophila, zebrafish, and mice. In C. elegans, RPM-1 (Regulator of Presynaptic Morphology-1) functions in synapse formation, axon guidance, axon termination, and postsynaptic GLR-1 trafficking. Acting as an E3 ubiquitin ligase, RPM-1 negatively regulates a MAP kinase pathway that includes: dlk-1, mkk-4, and the p38 MAPK, pmk-3. Here we provide evidence that ppm-1, a serine/threonine phosphatase homologous to human PP2Cα(PPM1A) and PP2Cβ(PPM1B) acts as a second negative regulatory mechanism to control the dlk-1 pathway. We show that ppm-1 functions through its phosphatase activity in a parallel genetic pathway with glo-4 and fsn-1 to regulate both synapse formation in the GABAergic motorneurons and axon termination in the mechanosensory neurons. Our transgenic analysis shows that ppm-1 acts downstream of rpm-1 to negatively regulate the DLK-1 pathway, with PPM-1 most likely acting at the level of pmk-3. Our study provides insight into the negative regulatory mechanisms that control the dlk-1 pathway in neurons and demonstrates a new role for the PP2C/PPM phosphatases as regulators of neuronal development. |
| 巻・号 | 189(4) |
| ページ | 1297-307 |
| 公開日 | 2011-12-1 |
| DOI | 10.1534/genetics.111.134791 |
| PII | genetics.111.134791 |
| PMID | 21968191 |
| PMC | PMC3241410 |
| MeSH | Animals Animals, Genetically Modified Axons* Caenorhabditis elegans / enzymology Caenorhabditis elegans / growth & development* Neurons / metabolism Phosphoprotein Phosphatases / metabolism* Synapses* |
| IF | 4.015 |
| 引用数 | 17 |
| WOS 分野 | GENETICS & HEREDITY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 2 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 線虫 | tm653 |