論文 - 詳細
| RRC ID | 46534 |
|---|---|
| 著者 | Xiao Y, Bedet C, Robert VJ, Simonet T, Dunkelbarger S, Rakotomalala C, Soete G, Korswagen HC, Strome S, Palladino F. |
| タイトル | Caenorhabditis elegans chromatin-associated proteins SET-2 and ASH-2 are differentially required for histone H3 Lys 4 methylation in embryos and adult germ cells. |
| ジャーナル | Proc Natl Acad Sci U S A |
| Abstract |
Methylation of histone H3 lysine 4 (H3K4me), a mark associated with gene activation, is mediated by SET1 and the related mixed lineage leukemia (MLL) histone methyltransferases (HMTs) across species. Mammals contain seven H3K4 HMTs, Set1A, Set1B, and MLL1-MLL5. The activity of SET1 and MLL proteins relies on protein-protein interactions within large multisubunit complexes that include three core components: RbBP5, Ash2L, and WDR5. It remains unclear how the composition and specificity of these complexes varies between cell types and during development. Caenorhabditis elegans contains one SET1 protein, SET-2, one MLL-like protein, SET-16, and single homologs of RbBP5, Ash2L, and WDR5. Here we show that SET-2 is responsible for the majority of bulk H3K4 methylation at all developmental stages. However, SET-2 and absent, small, or homeotic discs 2 (ASH-2) are differentially required for tri- and dimethylation of H3K4 (H3K4me3 and -me2) in embryos and adult germ cells. In embryos, whereas efficient H3K4me3 requires both SET-2 and ASH-2, H3K4me2 relies mostly on ASH-2. In adult germ cells by contrast, SET-2 serves a major role whereas ASH-2 is dispensable for H3K4me3 and most H3K4me2. Loss of SET-2 results in progressive sterility over several generations, suggesting an important function in the maintenance of a functional germ line. This study demonstrates that individual subunits of SET1-related complexes can show tissue specificity and developmental regulation and establishes C. elegans as a model to study SET1-related complexes in a multicellular organism. |
| 巻・号 | 108(20) |
| ページ | 8305-10 |
| 公開日 | 2011-5-17 |
| DOI | 10.1073/pnas.1019290108 |
| PII | 1019290108 |
| PMID | 21527717 |
| PMC | PMC3100940 |
| MeSH | Animals Caenorhabditis elegans / physiology* Caenorhabditis elegans Proteins / physiology* Embryo, Nonmammalian / metabolism* Germ Cells / metabolism* Histone-Lysine N-Methyltransferase / physiology* Histones / metabolism* Lysine / metabolism Methylation Nuclear Proteins / physiology* Saccharomyces cerevisiae Proteins / physiology |
| IF | 9.412 |
| 引用数 | 53 |
| WOS 分野 | MULTIDISCIPLINARY SCIENCES |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 各媒体での言及数の合計 | 0 |
| リソース情報 | |
| 線虫 | tm1905 |