RRC ID 4655
Author Tachibana H, Yanagi T, Akatsuka A, Kobayashi S, Kanbara H, Tsutsumi V.
Title Isolation and characterization of a potentially virulent species Entamoeba nuttalli from captive Japanese macaques.
Journal Parasitology
Abstract We have recently proposed revival of the name Entamoeba nuttalli Castellani, 1908 for a virulent amoeba (P19-061405 strain) isolated from a rhesus monkey (Macaca mulatta) and located phylogenetically between E. histolytica and E. dispar. In this study, E. nuttalli was isolated from feces of captive Japanese macaques (M. fuscata) in an open-air corral in Japan. The sequence of the 18S rRNA gene in the isolates differed from the P19-061405 strain in 2 nucleotide positions, but was identical to the EHMfas1 strain isolated previously from a cynomolgus monkey (M. fascicularis). One of the E. nuttalli isolates from Japanese macaques, named the NASA6 strain, was axenized and cloned. In isoenzyme analysis, the mobilities of hexokinase and phosphate glucose isomerase in the NASA6 strain were identical to those in the P19-061405 and EHMfas1 strains, but the mobility of phosphoglucomutase was different. These results were supported by gene analyses of these enzymes. Inoculation of NASA6 strain trophozoites into the liver of hamsters led to formation of an amoebic liver abscess. The liver lesions were characterized by extensive necrosis associated with inflammatory reactions. These results demonstrate that the NASA6 strain is potentially virulent and that E. nuttalli should be recognized as a common parasite in macaques.
Volume 136(10)
Pages 1169-77
Published 2009-9
DOI 10.1017/S0031182009990576
PII S0031182009990576
PMID 19635174
MeSH Animals Cricetinae Entamoeba / classification* Entamoeba / genetics Entamoeba / isolation & purification Entamoeba / pathogenicity* Entamoebiasis* / epidemiology Entamoebiasis* / parasitology Entamoebiasis* / pathology Feces / parasitology Isoenzymes / analysis Japan Liver / parasitology Liver / pathology Liver Abscess, Amebic / parasitology Liver Abscess, Amebic / pathology Macaca / classification Macaca / parasitology* Monkey Diseases* / epidemiology Monkey Diseases* / parasitology Monkey Diseases* / pathology RNA, Ribosomal, 18S / genetics Sequence Analysis, DNA Species Specificity Virulence
IF 2.456
Times Cited 18
Pathogenic microorganisms Entamoeba nuttalli NASA6