| RRC ID |
4655
|
| 著者 |
Tachibana H, Yanagi T, Akatsuka A, Kobayashi S, Kanbara H, Tsutsumi V.
|
| タイトル |
Isolation and characterization of a potentially virulent species Entamoeba nuttalli from captive Japanese macaques.
|
| ジャーナル |
Parasitology
|
| Abstract |
We have recently proposed revival of the name Entamoeba nuttalli Castellani, 1908 for a virulent amoeba (P19-061405 strain) isolated from a rhesus monkey (Macaca mulatta) and located phylogenetically between E. histolytica and E. dispar. In this study, E. nuttalli was isolated from feces of captive Japanese macaques (M. fuscata) in an open-air corral in Japan. The sequence of the 18S rRNA gene in the isolates differed from the P19-061405 strain in 2 nucleotide positions, but was identical to the EHMfas1 strain isolated previously from a cynomolgus monkey (M. fascicularis). One of the E. nuttalli isolates from Japanese macaques, named the NASA6 strain, was axenized and cloned. In isoenzyme analysis, the mobilities of hexokinase and phosphate glucose isomerase in the NASA6 strain were identical to those in the P19-061405 and EHMfas1 strains, but the mobility of phosphoglucomutase was different. These results were supported by gene analyses of these enzymes. Inoculation of NASA6 strain trophozoites into the liver of hamsters led to formation of an amoebic liver abscess. The liver lesions were characterized by extensive necrosis associated with inflammatory reactions. These results demonstrate that the NASA6 strain is potentially virulent and that E. nuttalli should be recognized as a common parasite in macaques.
|
| 巻・号 |
136(10)
|
| ページ |
1169-77
|
| 公開日 |
2009-9-1
|
| DOI |
10.1017/S0031182009990576
|
| PII |
S0031182009990576
|
| PMID |
19635174
|
| MeSH |
Animals
Cricetinae
Entamoeba / classification*
Entamoeba / genetics
Entamoeba / isolation & purification
Entamoeba / pathogenicity*
Entamoebiasis* / epidemiology
Entamoebiasis* / parasitology
Entamoebiasis* / pathology
Feces / parasitology
Isoenzymes / analysis
Japan
Liver / parasitology
Liver / pathology
Liver Abscess, Amebic / parasitology
Liver Abscess, Amebic / pathology
Macaca / classification
Macaca / parasitology*
Monkey Diseases* / epidemiology
Monkey Diseases* / parasitology
Monkey Diseases* / pathology
RNA, Ribosomal, 18S / genetics
Sequence Analysis, DNA
Species Specificity
Virulence
|
| IF |
2.783
|
| 引用数 |
32
|
|
WOS 分野
|
PARASITOLOGY
|
| リソース情報 |
| 病原微生物 |
Entamoeba nuttalli
NASA6 |
