論文 - 詳細
| RRC ID | 46613 |
|---|---|
| 著者 | Horikawa M, Sakamoto K. |
| タイトル | Polyunsaturated fatty acids are involved in regulatory mechanism of fatty acid homeostasis via daf-2/insulin signaling in Caenorhabditis elegans. |
| ジャーナル | Mol Cell Endocrinol |
| Abstract |
The development of the dauer form of Caenorhabditis elegans daf-2(e1370) enhances the expression of genes such as fatty acid desaturase fat-6 and fat-7 and fatty acid elongase elo-2, and increases the level of triglyceride (TAG). RNA interference (RNAi) of the fat-6, fat-7, and elo-2 genes lowers fat accumulation in the nematode. We recently clarified the fact that RNAi of fat-related genes, especially fat-2, reduced fat accumulation and activated DAF-16. FAT-2 regulates the first step of polyunsaturated fatty acid (PUFA) synthesis. RNAi of fat-2 induced nuclear translocation of DAF-16 and increased the level of TAG that could be detected by Oil Red-O, but suppressed the accumulation of lipid dyed by Nile red. TAG levels are also increased in the adult daf-2(e1370), whereas Nile red staining showed fat reduction. Introduction of RNAi of fat-2, fat-6, fat-7, and elo-2 genes into the daf-16 deficient worm recovered Nile red-stained lipid storage. These results suggest that Nile red stained the lipids except TAG, and that the levels of these lipids are regulated by daf-16. In fat-2, fat-6, fat-7, and elo-2-RNAi worms, the Nile red-stained fat level was restored through addition of fatty acids, especially PUFA. This suggests that reduction of Nile red-dyed lipid reflects the disorder of fatty acid metabolism. Furthermore, treatment of the fat-2-RNAi worm with PUFA--using the fatty acids from linoleic acid through eicosapentaenoic acid--suppressed nuclear localization of DAF-16. These results suggest that PUFA acts as a mediator of daf-2/insulin signaling and that daf-16 might be involved in fatty acid homeostasis under the control of PUFA. |
| 巻・号 | 323(2) |
| ページ | 183-92 |
| 公開日 | 2010-7-29 |
| DOI | 10.1016/j.mce.2010.03.004 |
| PII | S0303-7207(10)00137-1 |
| PMID | 20226839 |
| MeSH | Animals Caenorhabditis elegans / anatomy & histology Caenorhabditis elegans / physiology* Caenorhabditis elegans Proteins / genetics Caenorhabditis elegans Proteins / metabolism* Fatty Acid Desaturases / genetics Fatty Acid Desaturases / metabolism Fatty Acids, Unsaturated / metabolism* Forkhead Transcription Factors Homeostasis / physiology* Insulin / metabolism* RNA Interference Receptor, Insulin / genetics Receptor, Insulin / metabolism* Signal Transduction / physiology* Transcription Factors / genetics Transcription Factors / metabolism Transcription, Genetic |
| IF | 3.871 |
| 引用数 | 14 |
| WOS 分野 | ENDOCRINOLOGY & METABOLISM CELL BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 各媒体での言及数の合計 | 0 |
| リソース情報 | |
| 線虫 | tm789 tm420 tm331 tm326 |