RRC ID 4662
Author Yoshikawa H, Wu Z, Pandey K, Pandey BD, Sherchand JB, Yanagi T, Kanbara H.
Title Molecular characterization of Blastocystis isolates from children and rhesus monkeys in Kathmandu, Nepal.
Journal Vet. Parasitol.
Abstract To investigate the possible transmission of Blastocystis organisms between local rhesus monkeys and children in Kathmandu, Nepal, we compared the subtype (ST) and sequence of Blastocystis isolates from children with gastrointestinal symptoms and local rhesus monkeys. Twenty and 10 Blastocystis isolates were established from 82 and 10 fecal samples obtained from children and monkeys, respectively. Subtype analysis with seven sequence-tagged site (STS) primers indicated that the prevalence of Blastocystis sp. ST1, ST2 and ST3 was 20%, 20% and 60% in the child isolates, respectively. In contrast to human isolates, ST3 was not found in monkey isolates and the prevalence of ST1 and ST2 was 50% and 70%, respectively, including three mixed STs1 and 2 and one isolate not amplified by any STS primers, respectively. Since Blastocystis sp. ST2 has been reported as the most dominant genotype in the survey of Blastocystis infection among the various monkey species, sequence comparison of the 150bp variable region of the small subunit rRNA (SSU rRNA) gene was conducted among ST2 isolates of humans and monkeys. Sequence alignment of 24 clones developed from ST2 isolates of 4 humans and 4 monkeys showed three distinct subgroups, defined as ST2A, ST2B and ST2C. These three subgroups were shared between the child and monkey isolates. These results suggest that the local rhesus monkeys are a possible source of Blastocystis sp. ST2 infection of humans in Kathmandu.
Volume 160(3-4)
Pages 295-300
Published 2009-3-23
DOI 10.1016/j.vetpar.2008.11.029
PII S0304-4017(08)00674-2
PMID 19136214
MeSH Adolescent Animals Blastocystis / classification Blastocystis / isolation & purification* Blastocystis Infections / epidemiology Blastocystis Infections / parasitology Blastocystis Infections / transmission* Blastocystis Infections / veterinary* Child Child, Preschool DNA, Protozoan / chemistry DNA, Protozoan / genetics Disease Reservoirs / parasitology Disease Reservoirs / veterinary Feces / parasitology Female Genotype Humans Infant Macaca mulatta* / parasitology Male Molecular Sequence Data Monkey Diseases / epidemiology Monkey Diseases / parasitology Monkey Diseases / transmission* Nepal / epidemiology Phylogeny RNA, Ribosomal Sequence Alignment Sequence Analysis, DNA Sequence Homology, Nucleic Acid Sequence Tagged Sites Zoonoses*
IF 2.422
Times Cited 38
Pathogenic microorganisms Blastocystis sp.