論文 - 詳細
| RRC ID | 46650 |
|---|---|
| 著者 | Matus DQ, Li XY, Durbin S, Agarwal D, Chi Q, Weiss SJ, Sherwood DR. |
| タイトル | In vivo identification of regulators of cell invasion across basement membranes. |
| ジャーナル | Sci Signal |
| Abstract |
Cell invasion through basement membranes during development, immune surveillance, and metastasis remains poorly understood. To gain further insight into this key cellular behavior, we performed an in vivo screen for regulators of cell invasion through basement membranes, using the simple model of Caenorhabditis elegans anchor cell invasion, and identified 99 genes that promote invasion, including the genes encoding the chaperonin complex cct. Notably, most of these genes have not been previously implicated in invasive cell behavior. We characterized members of the cct complex and 11 other gene products, determining the distinct aspects of the invasive cascade that they regulate, including formation of a specialized invasive cell membrane and its ability to breach the basement membrane. RNA interference-mediated knockdown of the human orthologs of cct-5 and lit-1, which had not previously been implicated in cell invasion, reduced the invasiveness of metastatic carcinoma cells, suggesting that a conserved genetic program underlies cell invasion. These results increase our understanding of the genetic underpinnings of cell invasion and also provide new potential therapeutic targets to limit this behavior. |
| 巻・号 | 3(120) |
| ページ | ra35 |
| 公開日 | 2010-5-4 |
| DOI | 10.1126/scisignal.2000654 |
| PII | 3/120/ra35 |
| PMID | 20442418 |
| PMC | PMC3917318 |
| MeSH | Animals Basement Membrane / cytology* Caenorhabditis elegans / cytology* Caenorhabditis elegans / genetics Neoplasm Invasiveness* / genetics RNA Interference |
| IF | 6.467 |
| 引用数 | 39 |
| WOS 分野 | BIOCHEMISTRY & MOLECULAR BIOLOGY CELL BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | F1000Research |
| 各媒体での言及数の合計 | 1 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 線虫 | tm3042 tm3126 tm3082 |