RRC ID 46669
著者 Sasagawa Y, Yamanaka K, Saito-Sasagawa Y, Ogura T.
タイトル Caenorhabditis elegans UBX cofactors for CDC-48/p97 control spermatogenesis.
ジャーナル Genes Cells
Abstract UBX (ubiquitin regulatory X) domain-containing proteins act as cofactors for CDC-48/p97. CDC-48/p97 is essential for various cellular processes including retro-translocation in endoplasmic reticulum-associated degradation, homotypic membrane fusion, nuclear envelope assembly, degradation of ubiquitylated proteins, and cell cycle progression. CDC-48/p97-dependent processes are determined by differential binding of cofactors including UBX proteins, but the cellular functions of UBX proteins have not yet been elucidated, especially in multicellular organisms. Therefore, we investigated the functions of UBX family members using Caenorhabditis elegans, which expresses six UBX proteins, UBXN-1 to UBXN-6. All six UBXN proteins directly interacted with CDC-48.1 and CDC-48.2, and simultaneous knockdown of the expression of three genes, ubxn-1, ubxn-2 and ubxn-3, induced embryonic lethal and sterile phenotypes, but knockdown of either one or two did not. The sterile worms had a feminized germ-line phenotype, producing oocytes but no sperm. UBXN-1, UBXN-2 and UBXN-3 colocalized with CDC-48 in spermatocytes but not mature sperm. TRA-1A, which is a key factor in the sex determination pathway and inhibits spermatogenesis, accumulated in worms in which UBXN-1, UBXN-2 and UBXN-3 had been simultaneously knocked down. Taken together, these results suggest that UBXN-1, UBXN-2 and UBXN-3 are redundant cofactors for CDC-48/p97 and control spermatogenesis via the degradation of TRA-1A.
巻・号 15(12)
ページ 1201-15
公開日 2010-12-1
DOI 10.1111/j.1365-2443.2010.01454.x
PMID 20977550
MeSH Adaptor Proteins, Signal Transducing / chemistry Adaptor Proteins, Signal Transducing / metabolism Adenosine Triphosphatases / metabolism* Animals Caenorhabditis elegans / metabolism* Caenorhabditis elegans Proteins / chemistry* Caenorhabditis elegans Proteins / metabolism* Cell Cycle Proteins / metabolism* Protein Structure, Tertiary Spermatogenesis* Ubiquitin / metabolism* Valosin Containing Protein
IF 1.655
引用数 15
WOS 分野 GENETICS & HEREDITY CELL BIOLOGY
リソース情報
線虫 tm544 tm659 tm1180 tm2759