論文 - 詳細
| RRC ID | 46684 |
|---|---|
| 著者 | Updike DL, Hachey SJ, Kreher J, Strome S. |
| タイトル | P granules extend the nuclear pore complex environment in the C. elegans germ line. |
| ジャーナル | J Cell Biol |
| Abstract |
The immortal and totipotent properties of the germ line depend on determinants within the germ plasm. A common characteristic of germ plasm across phyla is the presence of germ granules, including P granules in Caenorhabditis elegans, which are typically associated with the nuclear periphery. In C. elegans, nuclear pore complex (NPC)-like FG repeat domains are found in the VASA-related P-granule proteins GLH-1, GLH-2, and GLH-4 and other P-granule components. We demonstrate that P granules, like NPCs, are held together by weak hydrophobic interactions and establish a size-exclusion barrier. Our analysis of intestine-expressed proteins revealed that GLH-1 and its FG domain are not sufficient to form granules, but require factors like PGL-1 to nucleate the localized concentration of GLH proteins. GLH-1 is necessary but not sufficient for the perinuclear location of granules in the intestine. Our results suggest that P granules extend the NPC environment in the germ line and provide insights into the roles of the PGL and GLH family proteins. |
| 巻・号 | 192(6) |
| ページ | 939-48 |
| 公開日 | 2011-3-21 |
| DOI | 10.1083/jcb.201010104 |
| PII | jcb.201010104 |
| PMID | 21402789 |
| PMC | PMC3063144 |
| MeSH | Animals Caenorhabditis elegans / cytology* Caenorhabditis elegans / metabolism Caenorhabditis elegans Proteins / genetics Caenorhabditis elegans Proteins / metabolism* Cytoplasmic Granules / metabolism* DEAD-box RNA Helicases / genetics DEAD-box RNA Helicases / metabolism* Germ Cells / cytology* Germ Cells / metabolism Protein Structure, Tertiary RNA-Binding Proteins / genetics RNA-Binding Proteins / metabolism* Recombinant Fusion Proteins / genetics Recombinant Fusion Proteins / metabolism |
| IF | 8.811 |
| 引用数 | 99 |
| WOS 分野 | CELL BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Blogs |
| 各媒体での言及数の合計 | 5 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 線虫 | tm1489 |