RRC ID 46684
Author Updike DL, Hachey SJ, Kreher J, Strome S.
Title P granules extend the nuclear pore complex environment in the C. elegans germ line.
Journal J Cell Biol
Abstract The immortal and totipotent properties of the germ line depend on determinants within the germ plasm. A common characteristic of germ plasm across phyla is the presence of germ granules, including P granules in Caenorhabditis elegans, which are typically associated with the nuclear periphery. In C. elegans, nuclear pore complex (NPC)-like FG repeat domains are found in the VASA-related P-granule proteins GLH-1, GLH-2, and GLH-4 and other P-granule components. We demonstrate that P granules, like NPCs, are held together by weak hydrophobic interactions and establish a size-exclusion barrier. Our analysis of intestine-expressed proteins revealed that GLH-1 and its FG domain are not sufficient to form granules, but require factors like PGL-1 to nucleate the localized concentration of GLH proteins. GLH-1 is necessary but not sufficient for the perinuclear location of granules in the intestine. Our results suggest that P granules extend the NPC environment in the germ line and provide insights into the roles of the PGL and GLH family proteins.
Volume 192(6)
Pages 939-48
Published 2011-3-21
DOI 10.1083/jcb.201010104
PII jcb.201010104
PMID 21402789
PMC PMC3063144
MeSH Animals Caenorhabditis elegans / cytology* Caenorhabditis elegans / metabolism Caenorhabditis elegans Proteins / genetics Caenorhabditis elegans Proteins / metabolism* Cytoplasmic Granules / metabolism* DEAD-box RNA Helicases / genetics DEAD-box RNA Helicases / metabolism* Germ Cells / cytology* Germ Cells / metabolism Protein Structure, Tertiary RNA-Binding Proteins / genetics RNA-Binding Proteins / metabolism* Recombinant Fusion Proteins / genetics Recombinant Fusion Proteins / metabolism
IF 8.811
Times Cited 99
C.elegans tm1489