RRC ID 4669
Author Günther C, Schlereth A, Udvardi M, Ott T.
Title Metabolism of reactive oxygen species is attenuated in leghemoglobin-deficient nodules of Lotus japonicus.
Journal Mol. Plant Microbe Interact.
Abstract Leghemoglobins together with high rates of respiration are believed to be major sources of reactive oxygen species (ROS) in root nodules of leguminous plants. High capacities of antioxidative systems apparently protect this organ from oxidative damage. Using leghemoglobin-RNA interference (LbRNAi) lines of Lotus japonicus, we found that loss of leghemoglobin results in significantly lower H(2)O(2) levels in nodules. Transcript levels and catalytic activities of ascorbate-glutathione cycle enzymes involved in H(2)O(2) detoxification as well as concentrations of reduced ascorbate were also altered in LbRNAi nodules. Thus, symbiotic leghemoglobins contribute significantly to ROS generation in functional nodules.
Volume 20(12)
Pages 1596-603
Published 2007-12
DOI 10.1094/MPMI-20-12-1596
PMID 17990967
MeSH Ascorbate Peroxidases Ascorbic Acid / metabolism Glutathione / metabolism Hydrogen Peroxide / metabolism Leghemoglobin / antagonists & inhibitors* Lotus / growth & development Lotus / metabolism* Peroxidases / metabolism RNA Interference RNA, Messenger / metabolism Reactive Oxygen Species / metabolism* Root Nodules, Plant / growth & development Root Nodules, Plant / metabolism*
IF 3.588
Times Cited 18
WOS Category PLANT SCIENCES BIOTECHNOLOGY & APPLIED MICROBIOLOGY BIOCHEMISTRY & MOLECULAR BIOLOGY
Resource
Lotus / Glycine Gifu B-129