論文 - 詳細
| RRC ID | 46714 |
|---|---|
| 著者 | Butschi A, Titz A, Wälti MA, Olieric V, Paschinger K, Nöbauer K, Guo X, Seeberger PH, Wilson IB, Aebi M, Hengartner MO, Künzler M. |
| タイトル | Caenorhabditis elegans N-glycan core beta-galactoside confers sensitivity towards nematotoxic fungal galectin CGL2. |
| ジャーナル | PLoS Pathog |
| Abstract |
The physiological role of fungal galectins has remained elusive. Here, we show that feeding of a mushroom galectin, Coprinopsis cinerea CGL2, to Caenorhabditis elegans inhibited development and reproduction and ultimately resulted in killing of this nematode. The lack of toxicity of a carbohydrate-binding defective CGL2 variant and the resistance of a C. elegans mutant defective in GDP-fucose biosynthesis suggested that CGL2-mediated nematotoxicity depends on the interaction between the galectin and a fucose-containing glycoconjugate. A screen for CGL2-resistant worm mutants identified this glycoconjugate as a Galbeta1,4Fucalpha1,6 modification of C. elegans N-glycan cores. Analysis of N-glycan structures in wild type and CGL2-resistant nematodes confirmed this finding and allowed the identification of a novel putative glycosyltransferase required for the biosynthesis of this glycoepitope. The X-ray crystal structure of a complex between CGL2 and the Galbeta1,4Fucalpha1,6GlcNAc trisaccharide at 1.5 A resolution revealed the biophysical basis for this interaction. Our results suggest that fungal galectins play a role in the defense of fungi against predators by binding to specific glycoconjugates of these organisms. |
| 巻・号 | 6(1) |
| ページ | e1000717 |
| 公開日 | 2010-1-1 |
| DOI | 10.1371/journal.ppat.1000717 |
| PMID | 20062796 |
| PMC | PMC2798750 |
| MeSH | Agaricales / immunology* Amino Acid Sequence Animals Caenorhabditis elegans / genetics Caenorhabditis elegans / immunology Caenorhabditis elegans / metabolism Caenorhabditis elegans Proteins / genetics Caenorhabditis elegans Proteins / immunology Caenorhabditis elegans Proteins / metabolism* Fungal Proteins / chemistry Fungal Proteins / immunology* Fungal Proteins / metabolism Galactosides / metabolism* Galectin 2 / chemistry Galectin 2 / immunology* Galectin 2 / metabolism Molecular Sequence Data Nematode Infections / immunology* Nematode Infections / metabolism Protein Structure, Quaternary Structure-Activity Relationship |
| IF | 6.218 |
| 引用数 | 69 |
| WOS 分野 | PARASITOLOGY MICROBIOLOGY VIROLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 3 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 線虫 | tm1078 |