RRC ID 46714
Author Butschi A, Titz A, Wälti MA, Olieric V, Paschinger K, Nöbauer K, Guo X, Seeberger PH, Wilson IB, Aebi M, Hengartner MO, Künzler M.
Title Caenorhabditis elegans N-glycan core beta-galactoside confers sensitivity towards nematotoxic fungal galectin CGL2.
Journal PLoS Pathog
Abstract The physiological role of fungal galectins has remained elusive. Here, we show that feeding of a mushroom galectin, Coprinopsis cinerea CGL2, to Caenorhabditis elegans inhibited development and reproduction and ultimately resulted in killing of this nematode. The lack of toxicity of a carbohydrate-binding defective CGL2 variant and the resistance of a C. elegans mutant defective in GDP-fucose biosynthesis suggested that CGL2-mediated nematotoxicity depends on the interaction between the galectin and a fucose-containing glycoconjugate. A screen for CGL2-resistant worm mutants identified this glycoconjugate as a Galbeta1,4Fucalpha1,6 modification of C. elegans N-glycan cores. Analysis of N-glycan structures in wild type and CGL2-resistant nematodes confirmed this finding and allowed the identification of a novel putative glycosyltransferase required for the biosynthesis of this glycoepitope. The X-ray crystal structure of a complex between CGL2 and the Galbeta1,4Fucalpha1,6GlcNAc trisaccharide at 1.5 A resolution revealed the biophysical basis for this interaction. Our results suggest that fungal galectins play a role in the defense of fungi against predators by binding to specific glycoconjugates of these organisms.
Volume 6(1)
Pages e1000717
Published 2010-1-1
DOI 10.1371/journal.ppat.1000717
PMID 20062796
PMC PMC2798750
MeSH Agaricales / immunology* Amino Acid Sequence Animals Caenorhabditis elegans / genetics Caenorhabditis elegans / immunology Caenorhabditis elegans / metabolism Caenorhabditis elegans Proteins / genetics Caenorhabditis elegans Proteins / immunology Caenorhabditis elegans Proteins / metabolism* Fungal Proteins / chemistry Fungal Proteins / immunology* Fungal Proteins / metabolism Galactosides / metabolism* Galectin 2 / chemistry Galectin 2 / immunology* Galectin 2 / metabolism Molecular Sequence Data Nematode Infections / immunology* Nematode Infections / metabolism Protein Structure, Quaternary Structure-Activity Relationship
IF 6.218
Times Cited 69
C.elegans tm1078