RRC ID 4682
Author Ishida K, Niwa Y, Yamashino T, Mizuno T.
Title A genome-wide compilation of the two-component systems in Lotus japonicus.
Journal DNA Res
Abstract The two-component systems (TCS), or histidine-to-aspartate phosphorelays, are evolutionarily conserved common signal transduction mechanisms that are implicated in a wide variety of cellular responses to environmental stimuli in both prokaryotes and eukaryotes including plants. Among higher plants, legumes including Lotus japonicus have a unique ability to engage in beneficial symbiosis with nitrogen-fixing bacteria. We previously presented a genome-wide compiled list of TCS-associated components of Mesorhizobium loti, which is a symbiont specific to L. japonicus (Hagiwara et al. 2004, DNA Res., 11, 57-65). To gain both general and specific insights into TCS of this currently attractive model legume, here we compiled TCS-associated components as many as possible from a genome-wide viewpoint by taking advantage that the efforts of whole genome sequencing of L. japonicus are almost at final stage. In the current database (http://www.kazusa.or.jp/lotus/index.html), it was found that L. japonicus has, at least, 14 genes each encoding a histidine kinase, 7 histidine-containing phosphotransmitter-related genes, 7 type-A response regulator (RR)-related genes, 11 type-B RR-related genes, and also 5 circadian clock-associated pseudo-RR genes. These results suggested that most of the L. japonicus TCS-associated genes have already been uncovered in this genome-wide analysis, if not all. Here, characteristics of these TCS-associated components of L. japonicus were inspected, one by one, in comparison with those of Arabidopsis thaliana. In addition, some critical experiments were also done to gain further insights into the functions of L. japonicus TCS-associated genes with special reference to cytokinin-mediated signal transduction and circadian clock.
Volume 16(4)
Pages 237-47
Published 2009-8-1
DOI 10.1093/dnares/dsp012
PII dsp012
PMID 19675111
PMC PMC2725789
MeSH Arabidopsis / genetics Arabidopsis / metabolism Circadian Rhythm Cytokinins / metabolism* Gene Expression Regulation, Plant* Genome, Plant* Lotus / genetics Lotus / metabolism* Lotus / physiology Plant Proteins* / genetics Plant Proteins* / metabolism Signal Transduction*
IF 4.009
Times Cited 26
Lotus / Glycine Lotus japonicus