| RRC ID |
46820
|
| Author |
Shigemura T, Nakazawa Y, Matsuda K, Sano K, Yaguchi T, Motobayashi M, Saito S, Noda S, Kobayashi N, Agematsu K, Honda T, Koike K.
|
| Title |
Serial monitoring of Mucorales DNA load in serum samples of a patient with disseminated mucormycosis after allogeneic bone marrow transplantation.
|
| Journal |
Int J Hematol
|
| Abstract |
Mucormycosis is a fatal complication in immunocompromised patients, and is additionally difficult to diagnose due to the lack of useful serum biomarkers. Using a quantitative PCR approach, we retrospectively analyzed Mucorales DNA load in sera collected serially from a 3-year-old patient with chronic granulomatous disease, who died of multi-organ failure probably due to dissemination of Rhizomucor pusillus, which was detected from necropsy specimens. Mucorales DNA load was below the detection limit on days 9, 2, and 4 after unrelated bone marrow transplantation. Rhizomucor DNA was first detected on day 14 (1.6 × 10(3) copies/mL), and subsequently fluctuated between 1.3 × 10(3) and 37.2 × 10(3) copies/mL until day 43. Rhizomucor achieved a peak value of 940.0 × 10(3) copies/mL on day 48 the day before death. The detection or fluctuation of Rhizomucor DNA appeared to be associated with corticosteroid dosages or C-reactive protein levels. This specific, noninvasive, and highly quantitative assay may be useful for the early diagnosis of mucormycosis and prediction of disease progression.
|
| Volume |
100(2)
|
| Pages |
206-9
|
| Published |
2014-8-1
|
| DOI |
10.1007/s12185-014-1597-8
|
| PMID |
24848632
|
| MeSH |
Bone Marrow Transplantation*
C-Reactive Protein / metabolism
Child, Preschool
DNA, Fungal / blood*
Fatal Outcome
Granulomatous Disease, Chronic / immunology*
Granulomatous Disease, Chronic / microbiology
Granulomatous Disease, Chronic / therapy
Humans
Immunocompromised Host*
Immunosuppressive Agents / adverse effects
Male
Mucormycosis / diagnosis
Mucormycosis / immunology*
Mucormycosis / microbiology
Mucormycosis / pathology
Rhizomucor / pathogenicity
Rhizomucor / physiology
Transplantation, Homologous
|
| IF |
2.245
|
| Times Cited |
9
|
|
WOS Category
|
HEMATOLOGY
|
| Resource |
| Pathogenic microorganisms |
|
