RRC ID 46827
Author Urayama S, Fukuhara T, Moriyama H, Toh-E A, Kawamoto S.
Title Heterologous expression of a gene of Magnaporthe oryzae chrysovirus 1 strain A disrupts growth of the human pathogenic fungus Cryptococcus neoformans.
Journal Microbiol. Immunol.
Abstract Magnaporthe oryzae chrysovirus 1 strain A (MoCV1-A) is the causal agent of growth repression and attenuated virulence (hypovirulence) of the rice blast fungus, M. oryzae. We have previously reported that heterologous expression of MoCV1-A ORF4 in Saccharomyces cerevisiae results in growth defects, a large central vacuole and other cytological changes. In this study, the effects of open reading frame (ORF) 4 expression in Cryptococcus neoformans, a human pathogenic fungus responsible for severe opportunistic infection, were investigated. Cells expressing the ORF4 gene in C. neoformans showed remarkably enlarged vacuoles, nuclear diffusion and a reduced growth rate. In addition, expression of ORF4 apparently suppressed formation of the capsule that surrounds the entire cell wall, which is one of the most important components of expression of virulence. After 5-fluoroorotic acid treatment of ORF4-expressing cells to remove the plasmid carrying the ORF4 gene, the resultant plasmid-free cells recovered normal morphology and growth, indicating that heterologous expression of the MoCV1-A ORF4 gene induces negative effects in C. neoformans. These data suggest that the ORF4 product is a candidate for a pharmaceutical protein to control disease caused by C. neoformans.
Volume 58(5)
Pages 294-302
Published 2014-5
DOI 10.1111/1348-0421.12148
PMID 24641700
MeSH Cryptococcus neoformans / cytology* Cryptococcus neoformans / genetics Cryptococcus neoformans / growth & development* Genes, Viral* Magnaporthe / virology* Organelles / ultrastructure RNA Viruses / genetics* RNA Viruses / isolation & purification Recombinant Proteins / biosynthesis* Recombinant Proteins / genetics Recombinant Proteins / toxicity*
IF 1.335
Times Cited 2
Pathogenic microorganisms ?