| RRC ID |
46849
|
| Author |
Ishikawa T, Itoh F, Yoshida S, Saijo S, Matsuzawa T, Gonoi T, Saito T, Okawa Y, Shibata N, Miyamoto T, Yamasaki S.
|
| Title |
Identification of distinct ligands for the C-type lectin receptors Mincle and Dectin-2 in the pathogenic fungus Malassezia.
|
| Journal |
Cell Host Microbe
|
| Abstract |
Various C-type lectin receptors (CLRs), including Mincle and Dectin-2, function as pattern recognition receptors and play a central role in immunity to fungal pathogens. However, the precise structures of the CLR ligands in various pathogenic fungi have yet to be completely defined. Here we report that Malassezia, an opportunistic skin fungal pathogen, is cooperatively recognized by Mincle and Dectin-2 through distinct ligands. Solvent-based fractionation revealed that Mincle and Dectin-2 recognize lipophilic and hydrophilic components of Malassezia, respectively. Mass spectrometry and nuclear magnetic resonance (NMR) revealed glyceroglycolipid and unique mannosyl fatty acids linked to mannitol as two Mincle ligands. An O-linked mannobiose-rich glycoprotein was identified as a Malassezia ligand for Dectin-2. Cytokine production in response to the Mincle ligands and the Dectin-2 ligand was abrogated in Mincle(-/-) and Dectin-2(-/-) dendritic cells, respectively. These results demonstrate that Mincle and Dectin-2 recognize distinct ligands in Malassezia to induce host immune responses.
|
| Volume |
13(4)
|
| Pages |
477-88
|
| Published |
2013-4-17
|
| DOI |
10.1016/j.chom.2013.03.008
|
| PII |
S1931-3128(13)00118-2
|
| PMID |
23601109
|
| MeSH |
Animals
Cytokines / immunology
Cytokines / metabolism
Fungi / immunology*
Fungi / metabolism
Glycolipids / immunology
Glycolipids / metabolism
Glycoproteins / immunology
Glycoproteins / metabolism
Lectins, C-Type / immunology*
Lectins, C-Type / metabolism
Ligands
Malassezia / immunology*
Malassezia / metabolism
Mannose / immunology
Mannose / metabolism
Membrane Proteins / immunology*
Membrane Proteins / metabolism
Mice
Mice, Inbred C57BL
Receptors, Mitogen / immunology*
Receptors, Mitogen / metabolism
|
| IF |
15.923
|
| Times Cited |
111
|
|
WOS Category
|
PARASITOLOGY
MICROBIOLOGY
VIROLOGY
|
| Resource |
| Pathogenic microorganisms |
IFM 48586
IFM 52635 |
