RRC ID 46853
Author Kopecká M, Kawamoto S, Yamaguchi M.
Title A new F-actin structure in fungi: actin ring formation around the cell nucleus of Cryptococcus neoformans.
Journal Microscopy (Oxf)
Abstract The F-actin cytoskeleton of Cryptococcus neoformans is known to comprise actin cables, cortical patches and cytokinetic ring. Here, we describe a new F-actin structure in fungi, a perinuclear F-actin collar ring around the cell nucleus, by fluorescent microscopic imaging of rhodamine phalloidin-stained F-actin. Perinuclear F-actin rings form in Cryptococcus neoformans treated with the microtubule inhibitor Nocodazole or with the drug solvent dimethyl sulfoxide (DMSO) or grown in yeast extract peptone dextrose (YEPD) medium, but they are absent in cells treated with Latrunculin A. Perinuclear F-actin rings may function as 'funicular cabin' for the cell nucleus, and actin cables as intracellular 'funicular' suspending nucleus in the central position in the cell and moving nucleus along the polarity axis along actin cables.
Volume 62(2)
Pages 295-301
Published 2013-4
DOI 10.1093/jmicro/dfs074
PII dfs074
PMID 23155113
MeSH Actin Cytoskeleton / ultrastructure* Actins / analysis* Bridged Bicyclo Compounds, Heterocyclic / pharmacology Cell Nucleus / ultrastructure* Cryptococcus neoformans / drug effects Cryptococcus neoformans / physiology Cryptococcus neoformans / ultrastructure* Dimethyl Sulfoxide / pharmacology Free Radical Scavengers / pharmacology Marine Toxins / pharmacology Microscopy, Electron Microscopy, Fluorescence Microtubules / drug effects Nocodazole / pharmacology Phalloidine / analogs & derivatives Rhodamines Thiazolidines / pharmacology Tubulin Modulators / pharmacology
IF 1.776
Times Cited 3
Pathogenic microorganisms IFM 40042 IFM 41464