Reference - Detail
|Author||Kopecká M, Yoshida S, Yamaguchi M.|
|Title||Actin ring formation around the cell nucleus of long-neck yeast.|
|Journal||J Electron Microsc (Tokyo)|
The unique long-neck yeast Fellomyces fuzhouensis has F-actin cables and cortical patches. Here, we describe a new F-actin structure present in fungi, a perinuclear F-actin collar ring around the cell nucleus. This F-actin structure can be visualized by fluorescent microscopic imaging of rhodamine-phalloidin-stained F-actin in cells treated with the mitotic drug isopropyl N-(3-chlorophenyl) carbamate or the microtubule inhibitor thiabendazol or when cells were grown in cut dried radish medium or yeast extract pepton dextrose (YEPD) medium. In contrast, these structures were absent in cells treated with Latrunculin A. The hypothetical functions of the F-actin ring are discussed.
|MeSH||Actin Cytoskeleton / metabolism Actin Cytoskeleton / ultrastructure* Actins / metabolism* Basidiomycota / drug effects Basidiomycota / growth & development Basidiomycota / metabolism* Basidiomycota / ultrastructure Bridged Bicyclo Compounds, Heterocyclic / pharmacology Cell Nucleus / metabolism* Microscopy, Fluorescence Thiazolidines / pharmacology|