| RRC ID |
4687
|
| Author |
Ichida H, Yoneyama K, Koba T, Abe T.
|
| Title |
Epigenetic modification of rhizobial genome is essential for efficient nodulation.
|
| Journal |
Biochem Biophys Res Commun
|
| Abstract |
CcrM is one of the solitary bacterial DNA methyltransferases which does not have corresponding restriction enzymes. We established a stable ccrM-overexpressing mutant of Mesorhizobium loti, MlccrM-OX, and performed molecular and phenotypic characterization of this strain. In the M. loti MlccrM-OX infected plants, nodulation was apparently delayed at 7 days after inoculation (dai), however, the nodules that eventually formed on the MlccrM-OX roots showed nitrogen fixing ability by at least 21 dai. These results suggest that the initial morphogenic events were affected by ccrM-overexpression and that the correct pattern of DNA methylation of the bacterial genome is not essential for plant-microbe symbiosis, but are required for efficient nodulation.
|
| Volume |
389(2)
|
| Pages |
301-4
|
| Published |
2009-11-13
|
| DOI |
10.1016/j.bbrc.2009.08.137
|
| PII |
S0006-291X(09)01734-3
|
| PMID |
19720053
|
| MeSH |
DNA Methylation
Epigenesis, Genetic*
Genome, Bacterial
Nitrogen Fixation / genetics*
Rhizobium / genetics*
Root Nodules, Plant / microbiology*
Site-Specific DNA-Methyltransferase (Adenine-Specific) / genetics*
Symbiosis
|
| IF |
2.985
|
| Times Cited |
10
|
|
WOS Category
|
BIOPHYSICS
BIOCHEMISTRY & MOLECULAR BIOLOGY
|
| Resource |
| Lotus / Glycine |
Miyakojima MG-20
M. loti |
