RRC ID 46959
Author Fredrich AL, Nagashima LA, Pavanelli WR, Marquez Ade S, Kaminami MS, Carlos Nde J, Sano A, Ono MA, Itano EN.
Title High molecular mass fraction in clinical isolates of Paracoccidioides brasiliensis.
Journal Rev Soc Bras Med Trop
Abstract INTRODUCTION:Different serum levels of the IgG/IgE for Paracoccidioides brasiliensis high mass molecular (hMM) fraction (~366 kDa) in the acute and chronic forms of the disease have been reported. Considering the nonexistence of hMM fraction investigation involving clinical isolates of P. brasiliensis, the present study aimed to investigate the presence of the hMM fraction (~366 kDa) in cell free antigens (CFA) from P. brasiliensis clinical isolates.
METHODS:CFA from 10 clinical isolates and a reference strain (Pb18) were submitted to SDS-polyacrylamide gel electrophoresis (SDS-PAGE) followed by gel image capturing and densitometer analysis. Additionally, CFA from 20 isolates and Pb18 were analyzed by capture ELISA (cELISA) using polyclonal (polAb) or monoclonal (mAb) antibodies to the hMM fraction.
RESULTS:The presence of the hMM component was observed in CFA of all samples analyzed by SDS-PAGE/densitometry and by cELISA. In addition, Pearson's correlation test demonstrated stronger coefficients between hMM fraction levels using pAb and mAb (R = 0.853) in cELISA.
CONCLUSIONS:The soluble hMM fraction was present in all the P. brasiliensis clinical isolates analyzed and the reference strain Pb18, which could be used as a source of this antigen. The work also introduces for first time, the cELISA method for P. brasiliensis hMM fraction detection. Analysis also suggests that detection is viable using polAb or mAb and this methodology may be useful for future investigation of the soluble hMM fraction (~366 kDa) in sera from PCM patients.
Volume 43(5)
Pages 526-30
Published 2010-1-1
DOI 10.1590/s0037-86822010000500011
PII S0037-86822010000500011
PMID 21085863
MeSH Antibodies, Fungal / immunology* Antibodies, Monoclonal / immunology* Antigens, Fungal / immunology* Chronic Disease Electrophoresis, Polyacrylamide Gel Enzyme-Linked Immunosorbent Assay Humans Immunoglobulin G / immunology* Molecular Weight Paracoccidioides / immunology* Paracoccidioides / isolation & purification Paracoccidioidomycosis / parasitology*
IF 1.339
Times Cited 1
Pathogenic microorganisms ?