RRC ID 46981
Author Virtudazo EV, Kawamoto S, Ohkusu M, Aoki S, Sipiczki M, Takeo K.
Title The single Cdk1-G1 cyclin of Cryptococcus neoformans is not essential for cell cycle progression, but plays important roles in the proper commitment to DNA synthesis and bud emergence in this yeast.
Journal FEMS Yeast Res.
Abstract The cell cycle pattern of the pathogenic basidiomycetous yeast Cryptococcus neoformans differs from that of the ascomycetous budding yeast Saccharomyces cerevisiae. To clarify the cell cycle control mechanisms at the molecular level, homologues of cell cycle control genes in C. neoformans were cloned and analyzed. Here, we report on the cloning and characterization of genes coding for CDK1 cyclin homologues, in particular, the C. neoformans G1 cyclin. We have identified three putative CDK1 cyclin homologues and two putative CDK5 (PHO85) cyclin homologues from the genome. Complementation tests in an S. cerevisiae G1 cyclin triple mutant confirmed that C. neoformans CLN1 is able to complement S. cerevisiae G1 cyclin deficiency, demonstrating that it is a G1 cyclin homologue. Interestingly, cells deleted of the single Cdk1-G1 cyclin were viable, demonstrating that this gene is not essential. However, it exhibited aberrant budding and cell division and a clear delay in the initiation of DNA synthesis as well as an extensive delay in budding. The fact that the mutant managed to traverse the G1 to M phase may be due to the activities of Pho85-related G1 cyclins. Also, that C. neoformans had only a single Cdk1-G1 cyclin highlighted the importance of keeping in order the commitment to the initiation of DNA synthesis first and then that of budding, as discussed.
Volume 10(5)
Pages 605-18
Published 2010-8-1
DOI 10.1111/j.1567-1364.2010.00633.x
PII FYR633
PMID 20528951
MeSH Amino Acid Sequence CDC2 Protein Kinase / genetics* CDC2 Protein Kinase / metabolism* Cell Cycle* Cloning, Molecular Computational Biology Cryptococcus neoformans / cytology Cryptococcus neoformans / enzymology* Cryptococcus neoformans / physiology* Cyclin-Dependent Kinase 5 / genetics Cyclin-Dependent Kinase 5 / metabolism DNA, Fungal / biosynthesis* Fungal Proteins / genetics Fungal Proteins / metabolism Gene Deletion Genetic Complementation Test Genome, Fungal Microbial Viability Molecular Sequence Data Saccharomyces cerevisiae / genetics Saccharomyces cerevisiae / growth & development Sequence Alignment Sequence Homology, Amino Acid
IF 2.609
Times Cited 7
WOS Category MYCOLOGY BIOTECHNOLOGY & APPLIED MICROBIOLOGY MICROBIOLOGY
Resource
Pathogenic microorganisms ?