| RRC ID |
46985
|
| 著者 |
Yamada H, Mitarai S, Chikamatsu K, Mizuno K, Yamaguchi M.
|
| タイトル |
Novel freeze-substitution electron microscopy provides new aspects of virulent Mycobacterium tuberculosis with visualization of the outer membrane and satisfying biosafety requirements.
|
| ジャーナル |
J Microbiol Methods
|
| Abstract |
Transmission electron microscopy (TEM) of virulent bacteria is usually performed following chemical fixation (CF) with aldehyde fixatives such as glutaraldehyde because of the biosafety problem. However, CF may alter sample ultrastructure. In this study, we used a rapid-freeze substitution (RFS) sandwich method without pre-embedding in agar. TEM images obtained using this method were completely different from those of conventional chemically fixed samples; the bacilli cytoplasm of the RFS preparations was filled evenly with numerous ribosomes, and there was no positional variation of electron density that was obvious in those obtained with CF samples. The sandwich method is suitable for microbiological materials without expensive devices and can be easily performed in a biosafety cabinet. In future, this method coupled with novel labeling techniques may help localize structural and functional molecules throughout a bacterial cell.
|
| 巻・号 |
80(1)
|
| ページ |
14-8
|
| 公開日 |
2010-1-1
|
| DOI |
10.1016/j.mimet.2009.09.022
|
| PII |
S0167-7012(09)00306-6
|
| PMID |
19799941
|
| MeSH |
Bacterial Outer Membrane Proteins / chemistry*
Bacterial Outer Membrane Proteins / ultrastructure
Fixatives / chemistry
Freeze Substitution / methods*
Glutaral / chemistry
Microscopy, Electron, Transmission / methods*
Mycobacterium tuberculosis / chemistry*
Mycobacterium tuberculosis / pathogenicity
Mycobacterium tuberculosis / ultrastructure
Virulence
|
| IF |
1.707
|
| 引用数 |
10
|
|
WOS 分野
|
BIOCHEMICAL RESEARCH METHODS
MICROBIOLOGY
|
| リソース情報 |
| 病原微生物 |
|
