| RRC ID |
4702
|
| 著者 |
Ito K, Katsuma S, Yamamoto K, Kadono-Okuda K, Mita K, Shimada T.
|
| タイトル |
A 25bp-long insertional mutation in the BmVarp gene causes the waxy translucent skin of the silkworm, Bombyx mori.
|
| ジャーナル |
Insect Biochem Mol Biol
|
| Abstract |
In Bombyx mori, there are more than 35 mutant strains whose larval skin color is transparent. The waxy translucent strain ow is one of the oily mutants which lack accumulation of uric acid in the epidermis. Here we performed positional cloning of the ow gene using the Bombyx draft genome sequence. For fine structure mapping, we succeeded to narrow the ow linked region to approximately 150kb, and identified the ow candidate gene by annotation analysis and DNA sequencing. The complete cDNA sequences of the ow gene from wild-type strains were 3501bp-long and potentially encoded a protein of 920 amino acids. We found a 25bp-long insertion in this gene in the ow mutant strain, resulting in a frame-shift mutation and generation of a premature stop codon. A BLAST search revealed that this protein had high homology to Varp, a recently identified protein containing a vacuolar sorting protein 9 domain and ankyrin repeats, and we termed the silkworm protein BmVarp. Varp has been shown to regulate endosome dynamics, suggesting that BmVarp may play an important role in the incorporation and/or accumulation of uric acid in the epidermis.
|
| 巻・号 |
39(4)
|
| ページ |
287-93
|
| 公開日 |
2009-4-1
|
| DOI |
10.1016/j.ibmb.2009.01.005
|
| PII |
S0965-1748(09)00007-1
|
| 解説 |
ow(ワクジー油）の原因遺伝子はBmVarpである。BmVarpはRAB38のエフェクターであるVarpのカイコホモログをコードする。w17系統（ow/ow)では、BmVarpのエクソン２に25bpの挿入配列が存在する。
|
| PMID |
19552891
|
| MeSH |
Animals
Base Sequence
Bombyx / chemistry*
Bombyx / classification
Bombyx / genetics*
Bombyx / metabolism
Gene Expression
Molecular Sequence Data
Mutagenesis, Insertional*
Phylogeny
Sequence Analysis, DNA
Skin / chemistry
Skin / metabolism
|
| IF |
3.827
|
| 引用数 |
22
|
|
WOS 分野
|
ENTOMOLOGY
BIOCHEMISTRY & MOLECULAR BIOLOGY
|
| リソース情報 |
| カイコ |
w17
u42
u49
w43 |
