| RRC ID |
47022
|
| Author |
Tatibana BT, Sano A, Uno J, Kamei K, Igarashi T, Mikami Y, Miyaji M, Nishimura K, Itano EN.
|
| Title |
Detection of Paracoccidioides brasiliensis gp43 gene in sputa by loop-mediated isothermal amplification method.
|
| Journal |
J Clin Lab Anal
|
| Abstract |
The fungus Paracoccidioides brasiliensis is the pathogen of paracoccidioidomycosis (PCM), a systemic mycosis prevalent in Latin America. The loop-mediated isothermal amplification method (LAMP) was used in this study to detect the presence of P. brasiliensis in sputa samples from patients with chronic PCM, suspected PCM, and a negative control. The target P. brasiliensis gp43 gene was amplified in less than 4 hr in 11 of 18 sputa samples tested. The LAMP method had the advantage of speed and simplicity compared with the classic diagnostic methods such as the histopathological test or biological material culture and did not require sophisticated technical apparatus. It would be an important aid in cases where immediate treatment would mean patient survival, especially in immune-suppressed patients.
|
| Volume |
23(2)
|
| Pages |
139-43
|
| Published |
2009-1-1
|
| DOI |
10.1002/jcla.20304
|
| PMID |
19288448
|
| PMC |
PMC6649160
|
| MeSH |
Adult
Aged
Antigens, Fungal / genetics*
Diagnostic Errors
Fungal Proteins / genetics*
Glycoproteins / genetics*
Humans
Male
Middle Aged
Nucleic Acid Amplification Techniques / methods*
Paracoccidioides / genetics
Paracoccidioides / isolation & purification*
Paracoccidioidomycosis / diagnosis*
Sensitivity and Specificity
Sputum / microbiology*
|
| IF |
1.54
|
| Times Cited |
15
|
|
WOS Category
|
MEDICAL LABORATORY TECHNOLOGY
|
| Resource |
| Pathogenic microorganisms |
IFM 41621 |
