| RRC ID |
47026
|
| Author |
Yamaguchi M, Okada H, Namiki Y.
|
| Title |
Smart specimen preparation for freeze substitution and serial ultrathin sectioning of yeast cells.
|
| Journal |
J Electron Microsc (Tokyo)
|
| Abstract |
A smart and efficient method for freeze substitution and serial sectioning of yeast cells is described. Yeast cells were placed in a single layer between two copper disks, rapidly frozen, freeze substituted and embedded in an epoxy resin. The cell layer was re-embedded by the same resin, the surface trimmed leaving 1 mum above the cell layer, and serially sectioned. The sections were collected on the two-slit grids and placed on a Formvar film mounted to cover the holes of an aluminum supporting rack. The grids were removed from the rack, stained together using a silicon tube and observed in a transmission electron microscope. The images of yeast cells observed were clear and natural, and would be useful for a detailed 3D structural analysis such as structome.
|
| Volume |
58(4)
|
| Pages |
261-6
|
| Published |
2009-8-1
|
| DOI |
10.1093/jmicro/dfp013
|
| PII |
dfp013
|
| PMID |
19289851
|
| MeSH |
Epoxy Resins
Freeze Substitution*
Microscopy, Electron, Transmission / methods*
Microtomy / methods*
Saccharomyces cerevisiae / ultrastructure*
Tissue Embedding*
|
| Times Cited |
38
|
|
WOS Category
|
MICROSCOPY
|
| Resource |
| Pathogenic microorganisms |
NA |
