| RRC ID |
47029
|
| Author |
Zeng J, de Souza Motta CM, Fukushima K, Takizawa K, Magalhes OM, Neves RP, Nishimura K.
|
| Title |
Identification of Trichosporon spp. strains by sequencing D1/D2 region and sub-typing by sequencing ribosomal intergenic spacer region of ribosomal DNA.
|
| Journal |
J Huazhong Univ Sci Technolog Med Sci
|
| Abstract |
To re-identify and further group 25 isolates of Trichosporon spp. identified morphologically previously, sequences of D1/D2 region of large subunit (LSU) of ribosomal DNA (rDNA) of 25 tested strains for identification and those of ribosomal intergenic space 1 (IGS1) region of 11 strains for subgrouping were detected. The identifications of tested strains were changed except 6 strains. According to the alignment of the IGS1 region, 6 T. asahii isolates tested fell into 4 groups and 5 T. faecale isolates into 3 groups. Polymorphism of 2 T. japonicum isolates was found in 10 positions. With the alignments obtained in this research compared with the relative GenBank entries, it was found that T. asahii, T. faecale and T. japonicum species were divided into 7, 3 and 2 subtypes respectively. Morphological and biophysical methods are not sufficient for Trichosporon spp. identification. Sequencing becomes necessary for Trichosporon diagnosis. There is obvious diversity within a species.
|
| Volume |
29(5)
|
| Pages |
655-8
|
| Published |
2009-10-1
|
| DOI |
10.1007/s11596-009-0524-3
|
| PMID |
19821104
|
| MeSH |
DNA, Fungal / chemistry
DNA, Fungal / genetics*
DNA, Ribosomal Spacer / chemistry
DNA, Ribosomal Spacer / genetics*
Genotype
Mycological Typing Techniques / methods
Sequence Analysis, DNA
Trichosporon / classification*
Trichosporon / genetics*
Trichosporon / isolation & purification
|
| IF |
1.151
|
| Times Cited |
6
|
|
WOS Category
|
BIOCHEMISTRY & MOLECULAR BIOLOGY
|
| Resource |
| Pathogenic microorganisms |
IFM 5780
IFM 5788
IFM 40067
IFM 41129
IFM 41606
IFM 48045
IFM 48552
IFM 48614
IFM 48619
IFM 48794
IFM 48951
IFM 49195
IFM 49226
IFM 50456
IFM 51753 |
