Reference - Detail
|Author||Myoken Y, Sugata T, Mikami Y, Murayama SY, Fujita Y.|
|Title||Identification of Aspergillus species in oral tissue samples of patients with hematologic malignancies by in situ hybridization: a preliminary report.|
|Journal||J. Oral Maxillofac. Surg.|
PURPOSE:A definitive diagnosis of invasive oral aspergillosis can be difficult because the culturing of tissue samples frequently fails to isolate Aspergillus species. In addition, the mycelial elements of Aspergillus species seen in tissue sections are histopathologically indistinguishable from those of non-Aspergillus species. We analyzed the usefulness of a DNA probe directed against the alkaline proteinase (ALP) gene of Aspergillus fumigatus for the identification of Aspergillus species by the in situ hybridization (ISH) technique in patients with oral mycosis.
PATIENTS AND METHODS:The ALP probe was tested on tissue specimens from 16 patients with hematologic malignancies who had invasive, orofacial fungal infections and a positive culture for one of the following organisms: Aspergillus species in 13 patients (A. flavus in 10, A. terreus in 2, and A. fumigatus in 1), and Exophiala dermatitis, Trichoderma longibrachiatum, and Candida albicans in 1 patient each. In situ hybridization with the ALP probe was performed using formalin-fixed, paraffin-embedded tissue samples.
RESULTS:The ALP probe showed a strong reaction with specimens from all 13 patients who had culture-proven aspergillosis specimens attributable to A. flavus, A. terreus, and A. fumigatus. On the other hand, the ALP probe showed no cross-reactivity with other fungi (Exophiala dermatitis, Trichoderma longibrachiatum, and Candida albicans).
CONCLUSION:These findings indicate that ISH using an ALP probe may increase the accuracy of diagnosing invasive oral aspergillosis in immunocompromised patients, and facilitate the provision of adequate antifungal treatment.
|MeSH||Adult Aged Alkaline Phosphatase / genetics Aspergillosis / complications Aspergillosis / diagnosis* Aspergillus / genetics Aspergillus / isolation & purification* DNA, Fungal / analysis Female Fungal Proteins / genetics Humans Immunocompromised Host In Situ Hybridization / methods* Leukemia / complications* Leukemia / drug therapy Leukemia / microbiology Male Middle Aged Neutropenia / chemically induced Neutropenia / complications Oligonucleotide Probes Retrospective Studies Sensitivity and Specificity Stomatitis / complications Stomatitis / microbiology*|
|WOS Category||DENTISTRY, ORAL SURGERY & MEDICINE|