RRC ID 47058
Author Fan SR, Bai FY, Liao QP, Liu ZH, Li J, Liu XP.
Title Genotype distribution of Candida albicans strains associated with different conditions of vulvovaginal candidiasis, as revealed by microsatellite typing.
Journal Sex Transm Infect
Abstract OBJECTIVE:To compare genotypes of Candida albicans strains causing different conditions of vulvovaginal candidiasis (VVC) in Chinese women.
METHODS:C albicans strains were isolated from the vaginas of patients with different conditions of VVC. The genotypes of the strains were investigated based on single-strand conformation polymorphisms (SSCP) of the PCR amplified microsatellite locus CAI.
RESULTS:A total of 93 independent C albicans strains was isolated from patients with mild-to-moderate (n = 37) or severe (n = 56) VVC. Thirty of the patients enrolled suffered recurrent VVC. Twenty-six distinct genotypes tentatively designated as A to Z were identified from the 93 C albicans strains compared on the basis of their CAI SSCP patterns. The majority (72.0%) of the strains possessed genotypes A to D, which were similar in the CAI SSCP profiles and were designated as the dominant genotypes. The overall frequencies of the four dominant genotypes were 87.5% (49/56) and 48.6% (18/37; p<0.001) in the C albicans strains from patients with severe and mild-to-moderate VVC, respectively. The strains with the dominant genotypes occupied 83.3% (25/30) and 66.7% (42/63; p = 0.094) in the C albicans strains from patients with recurrent VVC and sporadic VVC, respectively.
CONCLUSION:The frequency of C albicans strains with the dominant genotypes (A to D) from patients with severe VVC was significantly higher than that from patients with mild-to-moderate VVC, implying that the CAI genotype distribution of C albicans strains correlates with the severity of VVC.
Volume 84(2)
Pages 103-6
Published 2008-4-1
DOI 10.1136/sti.2007.025700
PII sti.2007.025700
PMID 17971371
MeSH Adult Candida albicans / genetics* Candidiasis, Vulvovaginal / genetics* Female Gene Frequency Genotype* Humans Microsatellite Repeats Middle Aged Polymorphism, Genetic / genetics Recurrence Vagina / microbiology*
IF 3.396
Times Cited 24
Pathogenic microorganisms NA