RRC ID 47179
Author Nakashima Y, Egami Y, Kimura M, Wakimoto T, Abe I.
Title Metagenomic Analysis of the Sponge Discodermia Reveals the Production of the Cyanobacterial Natural Product Kasumigamide by 'Entotheonella'.
Journal PLoS One
Abstract Sponge metagenomes are a useful platform to mine cryptic biosynthetic gene clusters responsible for production of natural products involved in the sponge-microbe association. Since numerous sponge-derived bioactive metabolites are biosynthesized by the symbiotic bacteria, this strategy may concurrently reveal sponge-symbiont produced compounds. Accordingly, a metagenomic analysis of the Japanese marine sponge Discodermia calyx has resulted in the identification of a hybrid type I polyketide synthase-nonribosomal peptide synthetase gene (kas). Bioinformatic analysis of the gene product suggested its involvement in the biosynthesis of kasumigamide, a tetrapeptide originally isolated from freshwater free-living cyanobacterium Microcystis aeruginosa NIES-87. Subsequent investigation of the sponge metabolic profile revealed the presence of kasumigamide in the sponge extract. The kasumigamide producing bacterium was identified as an 'Entotheonella' sp. Moreover, an in silico analysis of kas gene homologs uncovered the presence of kas family genes in two additional bacteria from different phyla. The production of kasumigamide by distantly related multiple bacterial strains implicates horizontal gene transfer and raises the potential for a wider distribution across other bacterial groups.
Volume 11(10)
Pages e0164468
Published 2016-1-1
DOI 10.1371/journal.pone.0164468
PII PONE-D-16-16842
PMID 27732651
PMC PMC5061366
MeSH Animals Cyanobacteria / enzymology Cyanobacteria / genetics* Cyanobacteria / physiology Gene Transfer, Horizontal Metagenome* Metagenomics Multigene Family Oligopeptides / genetics* Oligopeptides / metabolism Phylogeny Polyketide Synthases / genetics Polyketide Synthases / metabolism Porifera / genetics* Porifera / microbiology* Porifera / physiology Symbiosis*
IF 2.74
Times Cited 16
Algae NIES-87