RRC ID 4720
Author Kawakami N, Lee JM, Mon H, Kubo Y, Banno Y, Kawaguchi Y, Maenaka K, Park EY, Koga K, Kusakabe T.
Title Efficient protein expression in Bombyx mori larvae of the strain d17 highly sensitive to B. mori nucleopolyhedrovirus.
Journal Mol. Biotechnol.
Abstract The recombinant protein expression by Bombyx mori nucleopolyhedrovirus (BmNPV) infecting silkworm larvae or pupae may endow us with a potent system for the production of large eukaryotic proteins. However, the screening of silkworm strains ideally suited to this method has scarcely been conducted. In the present study, we injected recombinant BmNPV containing a reporter gene, luciferase or DsRed, into hemocoel of fifth instar larvae of selected 12 silkworm strains. Among them, the strain d17 is found to be the highest in reporter expression from the intrinsic polyhedrin promoter of Autographa californica NPV or the silkworm actin A3 promoter. These results suggest that the d17 strain is highly permissive for BmNPV replication and is the most likely candidate of a "factory" for large-scale expression using the BmNPV bacmid system.
Volume 40(2)
Pages 180-5
Published 2008-10
DOI 10.1007/s12033-008-9074-3
PMID 18543128
MeSH Animals Bombyx / classification Bombyx / genetics Bombyx / metabolism* Bombyx / virology* Gene Expression Genes, Reporter / genetics Larva / classification Larva / genetics Larva / metabolism Larva / virology Nucleopolyhedrovirus / genetics* Nucleopolyhedrovirus / physiology*
IF 1.815
Times Cited 15
Silkworms c11 d09 d17 e30 f38 k45 l312 n04 o46 p50 u11 w05