論文 - 詳細
| RRC ID | 47213 |
|---|---|
| 著者 | Nazifi E, Wada N, Asano T, Nishiuchi T, Iwamuro Y, Chinaka S, Matsugo S, Sakamoto T. |
| タイトル | Characterization of the chemical diversity of glycosylated mycosporine-like amino acids in the terrestrial cyanobacterium Nostoc commune. |
| ジャーナル | J Photochem Photobiol B |
| Abstract |
Mycosporine-like amino acids (MAAs) are UV-absorbing pigments, and structurally unique glycosylated MAAs are found in the terrestrial cyanobacterium Nostoc commune. In this study, we examined two genotypes of N.commune colonies with different water extract UV-absorption spectra. We found structurally distinct MAAs in each genotype. The water extract from genotype A showed a UV-absorbing spectrum with an absorption maximum at 335nm. The extract contained the following compounds: 7-O-(β-arabinopyranosyl)-porphyra-334 (478Da), pentose-bound shinorine (464Da), hexose-bound porphyra-334 (508Da) and porphyra-334 (346Da). The water extract from genotype B showed a characteristic UV-absorbing spectrum with double absorption maxima at 312 and 340nm. The extract contained hybrid MAAs (1050Da and 880Da) with two distinct chromophores of 3-aminocyclohexen-1-one and 1,3-diaminocyclohexen linked to 2-O-(β-xylopyranosyl)-β-galactopyranoside. A novel 273-Da MAA with an absorption maximum at 310nm was also identified in genotype B. The MAA consisted of a 3-aminocyclohexen-1-one linked to a γ-aminobutyric acid chain. These MAAs had potent radical scavenging activities in vitro and the results confirmed that the MAAs have multiple roles as a UV protectant and an antioxidant relevant to anhydrobiosis in N. commune. The two genotypes of N. commune exclusively produced their own characteristic glycosylated MAAs, which supports that MAA composition could be a chemotaxonomic marker for the classification of N. commune. |
| 巻・号 | 142 |
| ページ | 154-68 |
| 公開日 | 2015-1-1 |
| DOI | 10.1016/j.jphotobiol.2014.12.008 |
| PII | S1011-1344(14)00371-6 |
| PMID | 25543549 |
| MeSH | Antioxidants / metabolism Chlorophyll / analysis Chlorophyll A Chromatography, High Pressure Liquid Cyclohexanols / analysis* Cyclohexanols / isolation & purification Cyclohexanols / metabolism Cyclohexanones / analysis Electrophoresis, Capillary Genotype Glycine / analogs & derivatives Glycine / analysis Glycosylation Molecular Weight Nostoc commune / genetics Nostoc commune / metabolism* RNA, Ribosomal, 16S / genetics RNA, Ribosomal, 16S / metabolism Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization Spectrophotometry, Ultraviolet |
| IF | 4.383 |
| 引用数 | 20 |
| WOS 分野 | BIOPHYSICS BIOCHEMISTRY & MOLECULAR BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
|
| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 8 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 藻類 | NIES-2538 |