| RRC ID |
47213
|
| 著者 |
Nazifi E, Wada N, Asano T, Nishiuchi T, Iwamuro Y, Chinaka S, Matsugo S, Sakamoto T.
|
| タイトル |
Characterization of the chemical diversity of glycosylated mycosporine-like amino acids in the terrestrial cyanobacterium Nostoc commune.
|
| ジャーナル |
J Photochem Photobiol B
|
| Abstract |
Mycosporine-like amino acids (MAAs) are UV-absorbing pigments, and structurally unique glycosylated MAAs are found in the terrestrial cyanobacterium Nostoc commune. In this study, we examined two genotypes of N.commune colonies with different water extract UV-absorption spectra. We found structurally distinct MAAs in each genotype. The water extract from genotype A showed a UV-absorbing spectrum with an absorption maximum at 335nm. The extract contained the following compounds: 7-O-(β-arabinopyranosyl)-porphyra-334 (478Da), pentose-bound shinorine (464Da), hexose-bound porphyra-334 (508Da) and porphyra-334 (346Da). The water extract from genotype B showed a characteristic UV-absorbing spectrum with double absorption maxima at 312 and 340nm. The extract contained hybrid MAAs (1050Da and 880Da) with two distinct chromophores of 3-aminocyclohexen-1-one and 1,3-diaminocyclohexen linked to 2-O-(β-xylopyranosyl)-β-galactopyranoside. A novel 273-Da MAA with an absorption maximum at 310nm was also identified in genotype B. The MAA consisted of a 3-aminocyclohexen-1-one linked to a γ-aminobutyric acid chain. These MAAs had potent radical scavenging activities in vitro and the results confirmed that the MAAs have multiple roles as a UV protectant and an antioxidant relevant to anhydrobiosis in N. commune. The two genotypes of N. commune exclusively produced their own characteristic glycosylated MAAs, which supports that MAA composition could be a chemotaxonomic marker for the classification of N. commune.
|
| 巻・号 |
142
|
| ページ |
154-68
|
| 公開日 |
2015-1-1
|
| DOI |
10.1016/j.jphotobiol.2014.12.008
|
| PII |
S1011-1344(14)00371-6
|
| PMID |
25543549
|
| MeSH |
Antioxidants / metabolism
Chlorophyll / analysis
Chlorophyll A
Chromatography, High Pressure Liquid
Cyclohexanols / analysis*
Cyclohexanols / isolation & purification
Cyclohexanols / metabolism
Cyclohexanones / analysis
Electrophoresis, Capillary
Genotype
Glycine / analogs & derivatives
Glycine / analysis
Glycosylation
Molecular Weight
Nostoc commune / genetics
Nostoc commune / metabolism*
RNA, Ribosomal, 16S / genetics
RNA, Ribosomal, 16S / metabolism
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
Spectrophotometry, Ultraviolet
|
| IF |
4.383
|
| 引用数 |
20
|
|
WOS 分野
|
BIOPHYSICS
BIOCHEMISTRY & MOLECULAR BIOLOGY
|
| リソース情報 |
| 藻類 |
NIES-2538 |
