RRC ID 47235
Author Tanaka W, Ohmori Y, Ushijima T, Matsusaka H, Matsushita T, Kumamaru T, Kawano S, Hirano HY.
Title Axillary Meristem Formation in Rice Requires the WUSCHEL Ortholog TILLERS ABSENT1.
Journal Plant Cell
Abstract Axillary shoot formation is a key determinant of plant architecture. Formation of the axillary shoot is regulated by initiation of the axillary meristem or outgrowth of the axillary bud. Here, we show that rice (Oryza sativa) TILLERS ABSENT1 (TAB1; also known as Os WUS), an ortholog of Arabidopsis thaliana WUS, is required to initiate axillary meristem development. We found that formation of the axillary meristem in rice proceeds via a transient state, which we term the premeristem, characterized by the expression of OSH1, a marker of indeterminate cells in the shoot apical meristem. In the tab1-1 (wus-1) mutant, however, formation of the axillary meristem is arrested at various stages of the premeristem zone, and OSH1 expression is highly reduced. TAB1/WUS is expressed in the premeristem zone, where it shows a partially overlapping pattern with OSH1. It is likely, therefore, that TAB1 plays an important role in maintaining the premeristem zone and in promoting the formation of the axillary meristem by promoting OSH1 expression. Temporal expression patterns of WUSCHEL-RELATED HOMEOBOX4 (WOX4) indicate that WOX4 is likely to regulate meristem maintenance instead of TAB1 after establishment of the axillary meristem. Lastly, we show that the prophyll, the first leaf in the secondary axis, is formed from the premeristem zone and not from the axillary meristem.
Volume 27(4)
Pages 1173-84
Published 2015-4-1
DOI 10.1105/tpc.15.00074
PII tpc.15.00074
PMID 25841039
PMC PMC4558701
MeSH Gene Expression Regulation, Plant Meristem / genetics Meristem / metabolism* Oryza / genetics Oryza / metabolism* Plant Proteins / genetics Plant Proteins / metabolism* Protein Binding
IF 9.618
Times Cited 62
Rice Induced mutation lines