RRC ID 47253
Author Hisano H, Sato K.
Title Genomic regions responsible for amenability to Agrobacterium-mediated transformation in barley.
Journal Sci Rep
Abstract Different plant cultivars of the same genus and species can exhibit vastly different genetic transformation efficiencies. However, the genetic factors underlying these differences in transformation rate remain largely unknown. In barley, 'Golden Promise' is one of a few cultivars reliable for Agrobacterium-mediated transformation. By contrast, cultivar 'Haruna Nijo' is recalcitrant to genetic transformation. We identified genomic regions of barley important for successful transformation with Agrobacterium, utilizing the 'Haruna Nijo' × 'Golden Promise' F2 generation and genotyping by 124 genome-wide SNP markers. We observed significant segregation distortions of these markers from the expected 1:2:1 ratio toward the 'Golden Promise'-type in regions of chromosomes 2H and 3H, indicating that the alleles of 'Golden Promise' in these regions might contribute to transformation efficiency. The same regions, which we termed Transformation Amenability (TFA) regions, were also conserved in transgenic F2 plants generated from a 'Morex' × 'Golden Promise' cross. The genomic regions identified herein likely include necessary factors for Agrobacterium-mediated transformation in barley. The potential to introduce these loci into any haplotype of barley opens the door to increasing the efficiency of transformation for target alleles into any haplotype of barley by the TFA-based methods proposed in this report.
Volume 6
Pages 37505
Published 2016-11-22
DOI 10.1038/srep37505
PII srep37505
PMID 27874056
PMC PMC5118740
MeSH Agrobacterium / physiology* Alleles Chromosome Segregation / genetics Cloning, Molecular Crosses, Genetic Epistasis, Genetic Gene Frequency / genetics Genes, Plant Genetic Complementation Test Genetic Loci Genetic Markers Genome, Plant* Genotype Hordeum / genetics* Plants, Genetically Modified Polymorphism, Single Nucleotide / genetics Transformation, Genetic*
IF 4.011
Times Cited 1
Barley Haruna Nijo Golden Promise