論文 - 詳細
| RRC ID | 4727 |
|---|---|
| 著者 | Kawaoka S, Katsuma S, Daimon T, Isono R, Omuro N, Mita K, Shimada T. |
| タイトル | Functional analysis of four Gloverin-like genes in the silkworm, Bombyx mori. |
| ジャーナル | Arch Insect Biochem Physiol |
| Abstract |
To identify genes involved in the innate immunity of the silkworm Bombyx mori, we constructed a cDNA library from the fat body of Escherichia coli-challenged B. mori larvae. Based on the expressed sequence tag (EST) data and whole genome shotgun sequence analysis, we found four Gloverin-like genes, BmGlov1-4, in the Bombyx genome. Northern blot and RT-PCR analysis showed that BmGlov1-4 were induced in the larval fat body after an immune challenge by the injection of E. coli; however, less induction was observed after the injection of a yeast Candida albicans. In silico sequence analysis revealed the presence of a motif homologous to NF-kappaB binding site in the upstream region of each BmGlov gene. Moreover, we expressed recombinant BmGlov1-4 proteins using the baculovirus expression system, and found that all the recombinant BmGlov1-4 significantly inhibited the growth of E. coli. |
| 巻・号 | 67(2) |
| ページ | 87-96 |
| 公開日 | 2008-2-1 |
| DOI | 10.1002/arch.20223 |
| PMID | 18076111 |
| MeSH | Actins / analysis Actins / biosynthesis Amino Acid Sequence Animals Antimicrobial Cationic Peptides / biosynthesis Antimicrobial Cationic Peptides / pharmacology Base Sequence Blotting, Northern Bombyx / genetics* Bombyx / immunology Bombyx / microbiology Candida albicans / immunology DNA, Complementary / chemistry Escherichia coli / drug effects Escherichia coli / immunology Expressed Sequence Tags / chemistry Fat Body / immunology Fat Body / metabolism Gene Expression Regulation / immunology* Genome, Insect / genetics Intercellular Signaling Peptides and Proteins Larva / genetics Larva / immunology Larva / microbiology Molecular Sequence Data Phylogeny Promoter Regions, Genetic / genetics Proteins / chemistry Proteins / genetics* Proteins / pharmacology Recombinant Proteins / analysis Recombinant Proteins / biosynthesis Recombinant Proteins / pharmacology Reverse Transcriptase Polymerase Chain Reaction Sequence Alignment Time Factors |
| IF | 1.536 |
| 引用数 | 43 |
| WOS 分野 | ENTOMOLOGY PHYSIOLOGY BIOCHEMISTRY & MOLECULAR BIOLOGY |
| リソース情報 | |
| カイコ | |
| 一般微生物 | JCM5491 JCM1621 |