RRC ID 4727
Author Kawaoka S, Katsuma S, Daimon T, Isono R, Omuro N, Mita K, Shimada T.
Title Functional analysis of four Gloverin-like genes in the silkworm, Bombyx mori.
Journal Arch Insect Biochem Physiol
Abstract To identify genes involved in the innate immunity of the silkworm Bombyx mori, we constructed a cDNA library from the fat body of Escherichia coli-challenged B. mori larvae. Based on the expressed sequence tag (EST) data and whole genome shotgun sequence analysis, we found four Gloverin-like genes, BmGlov1-4, in the Bombyx genome. Northern blot and RT-PCR analysis showed that BmGlov1-4 were induced in the larval fat body after an immune challenge by the injection of E. coli; however, less induction was observed after the injection of a yeast Candida albicans. In silico sequence analysis revealed the presence of a motif homologous to NF-kappaB binding site in the upstream region of each BmGlov gene. Moreover, we expressed recombinant BmGlov1-4 proteins using the baculovirus expression system, and found that all the recombinant BmGlov1-4 significantly inhibited the growth of E. coli.
Volume 67(2)
Pages 87-96
Published 2008-2-1
DOI 10.1002/arch.20223
PMID 18076111
MeSH Actins / analysis Actins / biosynthesis Amino Acid Sequence Animals Antimicrobial Cationic Peptides / biosynthesis Antimicrobial Cationic Peptides / pharmacology Base Sequence Blotting, Northern Bombyx / genetics* Bombyx / immunology Bombyx / microbiology Candida albicans / immunology DNA, Complementary / chemistry Escherichia coli / drug effects Escherichia coli / immunology Expressed Sequence Tags / chemistry Fat Body / immunology Fat Body / metabolism Gene Expression Regulation / immunology* Genome, Insect / genetics Intercellular Signaling Peptides and Proteins Larva / genetics Larva / immunology Larva / microbiology Molecular Sequence Data Phylogeny Promoter Regions, Genetic / genetics Proteins / chemistry Proteins / genetics* Proteins / pharmacology Recombinant Proteins / analysis Recombinant Proteins / biosynthesis Recombinant Proteins / pharmacology Reverse Transcriptase Polymerase Chain Reaction Sequence Alignment Time Factors
IF 1.536
Times Cited 43
General Microbes JCM5491 JCM1621