RRC ID 47279
Author Oda-Ishii I, Kubo A, Kari W, Suzuki N, Rothbächer U, Satou Y.
Title A Maternal System Initiating the Zygotic Developmental Program through Combinatorial Repression in the Ascidian Embryo.
Journal PLoS Genet.
Abstract Maternal factors initiate the zygotic developmental program in animal embryos. In embryos of the chordate, Ciona intestinalis, three maternal factors-Gata.a, β-catenin, and Zic-r.a-are required to establish three domains of gene expression at the 16-cell stage; the animal hemisphere, vegetal hemisphere, and posterior vegetal domains. Here, we show how the maternal factors establish these domains. First, only β-catenin and its effector transcription factor, Tcf7, are required to establish the vegetal hemisphere domain. Second, genes specifically expressed in the posterior vegetal domain have additional repressive cis-elements that antagonize the activity of β-catenin/Tcf7. This antagonizing activity is suppressed by Zic-r.a, which is specifically localized in the posterior vegetal domain and binds to DNA indirectly through the interaction with Tcf7. Third, Gata.a directs specific gene expression in the animal hemisphere domain, because β-catenin/Tcf7 weakens the Gata.a-binding activity for target sites through a physical interaction in the vegetal cells. Thus, repressive regulation through protein-protein interactions among the maternal transcription factors is essential to establish the first distinct domains of gene expression in the chordate embryo.
Volume 12(5)
Pages e1006045
Published 2016-5
DOI 10.1371/journal.pgen.1006045
PMID 27152625
PMC PMC4859511
MeSH Animals Blastomeres / metabolism Body Patterning / genetics Ciona intestinalis / genetics Ciona intestinalis / growth & development Embryonic Development / genetics* GATA Transcription Factors / biosynthesis GATA Transcription Factors / genetics Gene Expression Regulation, Developmental In Situ Hybridization Protein Interaction Maps Sequence Analysis, DNA T Cell Transcription Factor 1 / biosynthesis T Cell Transcription Factor 1 / genetics* Zygote / growth & development* Zygote / metabolism beta Catenin / biosynthesis beta Catenin / genetics*
IF 5.54
Times Cited 5
C.intestinalis / (O.japonicus) Wild C. int