論文 - 詳細
| RRC ID | 47455 |
|---|---|
| 著者 | Chechenova MB, Maes S, Oas ST, Nelson C, Kiani KG, Bryantsev AL, Cripps RM. |
| タイトル | Functional redundancy and nonredundancy between two Troponin C isoforms in Drosophila adult muscles. |
| ジャーナル | Mol Biol Cell |
| Abstract |
We investigated the functional overlap of two muscle Troponin C (TpnC) genes that are expressed in the adult fruit fly, Drosophila melanogaster: TpnC4 is predominantly expressed in the indirect flight muscles (IFMs), whereas TpnC41C is the main isoform in the tergal depressor of the trochanter muscle (TDT; jump muscle). Using CRISPR/Cas9, we created a transgenic line with a homozygous deletion of TpnC41C and compared its phenotype to a line lacking functional TpnC4 We found that the removal of either of these genes leads to expression of the other isoform in both muscle types. The switching between isoforms occurs at the transcriptional level and involves minimal enhancers located upstream of the transcription start points of each gene. Functionally, the two TpnC isoforms were not equal. Although ectopic TpnC4 in TDT muscles was able to maintain jumping ability, TpnC41C in IFMs could not effectively support flying. Simultaneous functional disruption of both TpnC genes resulted in jump-defective and flightless phenotypes of the survivors, as well as abnormal sarcomere organization. These results indicated that TpnC is required for myofibril assembly, and that there is functional specialization among TpnC isoforms in Drosophila. |
| 巻・号 | 28(6) |
| ページ | 760-770 |
| 公開日 | 2017-3-15 |
| DOI | 10.1091/mbc.E16-07-0498 |
| PII | mbc.E16-07-0498 |
| PMID | 28077621 |
| PMC | PMC5349783 |
| MeSH | Animals Drosophila melanogaster / metabolism Muscle, Skeletal / metabolism Muscle, Skeletal / physiology* Muscles / metabolism Protein Isoforms / metabolism Troponin C / genetics Troponin C / metabolism* Troponin C / physiology* |
| IF | 3.791 |
| 引用数 | 8 |
| WOS 分野 | CELL BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 1 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ショウジョウバエ | CAS-0002 |