RRC ID 47485
Author Srinivasan R, Mishra M, Leong FY, Chiam KH, Balasubramanian M.
Title Bacillus anthracis tubulin-related protein Ba-TubZ assembles force-generating polymers.
Journal Cytoskeleton (Hoboken)
Abstract Pathogenicity of Bacillus anthracis depends on the faithful inheritance of plasmid pXO1, in a process that requires the plasmid encoded tubulin-related protein Ba-TubZ. Here we show, using heterologous expression in Schizosaccharomyces pombe, that Ba-TubZ assembles into a dynamic polymer in the absence of other B. anthracis proteins and can generate force capable of deforming the fission yeast nuclear envelope. The polymer bundles contain 27 ± 15 protofilaments/μm assuming that each protofilament spans the entire length. Thinner appearing buckled and thicker appearing straight filaments of Ba-TubZ were both capable of inducing nuclear envelope deformation. Unlike the related protein Bt-TubZ from Bacillus thuringiensis, which undergoes treadmilling upon expression in fission yeast, Ba-TubZ polymers did not undergo detectable treadmilling. Instead, in fluorescence recovery after photobleaching experiments, it displayed a different turnover behavior characterized by moderate fluorescence recovery along the entire length of the polymer. Modeling Ba-TubZ bundles as Euler-Bernoulli beams that buckle under compressive loads when pushed against the nuclear envelope allowed us to estimate that Ba-TubZ generates forces in the order of 1-10 nN. We propose that polymerization based filament elongation and force generation might aid faithful segregation of the virulence plasmid.
Volume 68(9)
Pages 501-11
Published 2011-9-1
DOI 10.1002/cm.20526
PMID 21780309
MeSH Bacillus anthracis / genetics Bacillus anthracis / metabolism* Bacillus anthracis / pathogenicity Bacillus thuringiensis / genetics Bacillus thuringiensis / metabolism Bacterial Proteins / genetics Bacterial Proteins / metabolism* Nuclear Envelope / genetics Nuclear Envelope / metabolism Plasmids / genetics Plasmids / metabolism Protein Multimerization / physiology* Schizosaccharomyces / genetics Schizosaccharomyces / metabolism
IF 2.72
Times Cited 2
WOS Category CELL BIOLOGY
Resource
DNA material pDUAL-HFG1c (RDB06133) pDUAL-HFG41c (RDB06134) pHFG41-ccdB2 (RDB06526).