Reference - Detail
| RRC ID | 47506 |
|---|---|
| Author | Satoh K, Maeda M, Umeda Y, Miyajima Y, Makimura K. |
| Title | Detection and identification of probable endemic fungal pathogen, Cryptococcus gattii, and worldwide pathogen, Cryptococcus neoformans, by real-time PCR. |
| Journal | Microbiol Immunol |
| Abstract |
A real-time PCR method for detection and identification of Cryptococcus neoformans and Cryptococcus gattii was developed and evaluated using DNA from single-colony or koala nasal smears. Two TaqMan minor groove binder probes that distinguished between these species were designed corresponding to the internal sequences of the CAP59 gene for both species. The real-time PCR assay had 100% specificity, as assessed using 13 reference strains and 300 environmental strains. Twelve smear samples from healthy koalas were analyzed by direct real-time PCR. This method successfully detected C. gattii and C. neoformans in one and three koalas, respectively. |
| Volume | 55(6) |
| Pages | 454-7 |
| Published | 2011-6-1 |
| DOI | 10.1111/j.1348-0421.2011.00324.x |
| PMID | 21623899 |
| MeSH | Animals Cryptococcus gattii / genetics Cryptococcus gattii / isolation & purification* Cryptococcus neoformans / genetics Cryptococcus neoformans / isolation & purification* Environmental Microbiology Fungal Proteins / genetics Mycology / methods* Nasal Mucosa / microbiology Oligonucleotide Probes / genetics Phascolarctidae / microbiology* Polymerase Chain Reaction / methods* Sensitivity and Specificity |
| IF | 1.566 |
| Times Cited | 9 |
| WOS Category | MICROBIOLOGY IMMUNOLOGY |
| Altmetric score |
オルトメトリクス指標項目
|
| The most frequently cited source | Patent(IFI CLAIMS) |
| Total number of mentions | 5 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| General Microbes | JCM 2466 JCM 15063 |