| RRC ID |
47528
|
| 著者 |
López JR, Hamman-Khalifa AM, Navas JI, de la Herran R.
|
| タイトル |
Characterization of ISR region and development of a PCR assay for rapid detection of the fish pathogen Tenacibaculum soleae.
|
| ジャーナル |
FEMS Microbiol Lett
|
| Abstract |
The aims of this work were to characterize the 16S-23S internal spacer region of the fish pathogen Tenacibaculum soleae and to develop a PCR assay for its identification and detection. All T. soleae strains tested displayed a single internal spacer region class, containing tRNA(I) (le) and tRNA(A) (la) genes; nevertheless, a considerable intraspecific heterogeneity was observed. However, this region proved to be useful for differentiation of T. soleae from related and non-related species. Species-specific primers were designed targeting the 16S rRNA gene and the internal spacer region region, yielding a 1555-bp fragment. Detection limit was of 1 pg DNA per reaction (< 30 bacterial cells) when using pure cultures. The detection level in the presence of DNA from fish or other bacteria was lower; however, 10 pg were detected at a target/background ratio of 1 : 10(5) . The PCR assay proved to be more sensitive than agar cultivation for the detection of T. soleae from naturally diseased fish, offering a useful tool for diagnosis and for understanding the epidemiology of this pathogen.
|
| 巻・号 |
324(2)
|
| ページ |
181-8
|
| 公開日 |
2011-11-1
|
| DOI |
10.1111/j.1574-6968.2011.02404.x
|
| PMID |
22092820
|
| MeSH |
Animals
Bacterial Typing Techniques
Base Sequence
DNA, Bacterial / genetics
DNA, Ribosomal Spacer / genetics*
Fish Diseases / microbiology*
Fishes / microbiology
Flavobacteriaceae Infections / microbiology
Flavobacteriaceae Infections / veterinary*
Molecular Sequence Data
Polymerase Chain Reaction / methods*
RNA, Ribosomal, 16S / genetics
RNA, Ribosomal, 23S / genetics
Tenacibaculum / classification
Tenacibaculum / genetics*
Tenacibaculum / isolation & purification*
|
| IF |
1.987
|
| 引用数 |
8
|
|
WOS 分野
|
MICROBIOLOGY
|
| リソース情報 |
| 一般微生物 |
JCM 13039 |
