論文 - 詳細
| RRC ID | 47528 |
|---|---|
| 著者 | López JR, Hamman-Khalifa AM, Navas JI, de la Herran R. |
| タイトル | Characterization of ISR region and development of a PCR assay for rapid detection of the fish pathogen Tenacibaculum soleae. |
| ジャーナル | FEMS Microbiol Lett |
| Abstract |
The aims of this work were to characterize the 16S-23S internal spacer region of the fish pathogen Tenacibaculum soleae and to develop a PCR assay for its identification and detection. All T. soleae strains tested displayed a single internal spacer region class, containing tRNA(I) (le) and tRNA(A) (la) genes; nevertheless, a considerable intraspecific heterogeneity was observed. However, this region proved to be useful for differentiation of T. soleae from related and non-related species. Species-specific primers were designed targeting the 16S rRNA gene and the internal spacer region region, yielding a 1555-bp fragment. Detection limit was of 1 pg DNA per reaction (< 30 bacterial cells) when using pure cultures. The detection level in the presence of DNA from fish or other bacteria was lower; however, 10 pg were detected at a target/background ratio of 1 : 10(5) . The PCR assay proved to be more sensitive than agar cultivation for the detection of T. soleae from naturally diseased fish, offering a useful tool for diagnosis and for understanding the epidemiology of this pathogen. |
| 巻・号 | 324(2) |
| ページ | 181-8 |
| 公開日 | 2011-11-1 |
| DOI | 10.1111/j.1574-6968.2011.02404.x |
| PMID | 22092820 |
| MeSH | Animals Bacterial Typing Techniques Base Sequence DNA, Bacterial / genetics DNA, Ribosomal Spacer / genetics* Fish Diseases / microbiology* Fishes / microbiology Flavobacteriaceae Infections / microbiology Flavobacteriaceae Infections / veterinary* Molecular Sequence Data Polymerase Chain Reaction / methods* RNA, Ribosomal, 16S / genetics RNA, Ribosomal, 23S / genetics Tenacibaculum / classification Tenacibaculum / genetics* Tenacibaculum / isolation & purification* |
| IF | 1.987 |
| 引用数 | 8 |
| WOS 分野 | MICROBIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
|
| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 1 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 一般微生物 | JCM 13039 |