論文 - 詳細
| RRC ID | 47534 |
|---|---|
| 著者 | Sakamoto T, Tanaka H, Nishimura Y, Ishimaru M, Kasai N. |
| タイトル | Characterization of an exo-β-1,3-D: -galactanase from Sphingomonas sp. 24T and its application to structural analysis of larch wood arabinogalactan. |
| ジャーナル | Appl Microbiol Biotechnol |
| Abstract |
A type II arabinogalactan-degrading enzyme, termed Exo-1,3-Gal, was purified to homogeneity from the culture filtrate of Sphingomonas sp. 24T. It has an apparent molecular mass of 48 kDa by SDS-PAGE. Exo-1,3-Gal was stable from pH 3 to 10 and at temperatures up to 40 °C. The optimum pH and temperature for enzyme activity were pH 6 to 7 and 50 °C, respectively. Galactose was released from β-1,3-D: -galactan and β-1,3-D: -galactooligosaccharides by the action of Exo-1,3-Gal, indicating that the enzyme was an exo-β-1,3-D: -galactanase. Analysis of the reaction products of β-1,3-galactotriose by high-performance anion-exchange chromatography revealed that the enzyme hydrolyzed the substrate in a non-processive mode. Exo-1,3-Gal bypassed the branching points of β-1,3-galactan backbones in larch wood arabinogalactan (LWAG) to produce mainly galactose, β-1,6-galactobiose, and unidentified oligosaccharides 1 and 2 with the molar ratios of 7:19:62:12. Oligosaccharides 1 and 2 were enzymatically determined to be β-1,6-galactotriose and β-1,6-galactotriose substituted with a single arabinofuranose residue, respectively. The ratio of side chains enzymatically released from LWAG was in good agreement with the postulated structure of the polysaccharide previously determined by chemical methods. |
| 巻・号 | 90(5) |
| ページ | 1701-10 |
| 公開日 | 2011-6-1 |
| DOI | 10.1007/s00253-011-3219-1 |
| PMID | 21452032 |
| MeSH | Bacterial Proteins / chemistry* Bacterial Proteins / genetics Bacterial Proteins / isolation & purification Bacterial Proteins / metabolism Enzyme Stability Galactans / chemistry* Galactans / metabolism Glycoside Hydrolases / chemistry* Glycoside Hydrolases / genetics Glycoside Hydrolases / isolation & purification Glycoside Hydrolases / metabolism Kinetics Larix / chemistry* Molecular Sequence Data Sphingomonas / chemistry Sphingomonas / enzymology* Sphingomonas / genetics Substrate Specificity Wood / chemistry* |
| IF | 3.53 |
| 引用数 | 9 |
| WOS 分野 | BIOTECHNOLOGY & APPLIED MICROBIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 各媒体での言及数の合計 | 0 |
| リソース情報 | |
| 一般微生物 | JCM 17462 |