RRC ID 47549
著者 Sato H, Teramoto K, Ishii Y, Watanabe K, Benno Y.
タイトル Ribosomal protein profiling by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry for phylogenety-based subspecies resolution of Bifidobacterium longum.
ジャーナル Syst Appl Microbiol
Abstract The taxonomic positions of the subspecies of Bifidobacterium longum (B. longum subsp. longum, subsp. infantis, and subsp. suis) have been controversial. A current proposal is that the former two species "B. infantis" and "B. suis" be unified with B. longum and all three reclassified as three subspecies. To test this proposal, ribosomal protein profiling as observed by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) was applied to the classification of 17 strains of B. longum, including three subspecies. Among 41 different kinds of ribosomal proteins selected as biomarkers whose masses were calculated from their amino acid sequences, 31-41 ribosomal proteins were observed in sample strains with the same masses as the references. The high matching rate indicates high conservation of ribosomal proteins within the sample strains, and therefore strongly supports the unification of the former species. However, the masses of some ribosomal proteins varied within species. The phylogenetic tree constructed from the profiles of ribosomal proteins matched the references, showing a clear cluster of the subsp. longum and the subsp. infantis strains. This result supports the proposal to reclassify B. longum into subsp. longum and subsp. infantis. The subsp. suis strains formed an individual sub-cluster within the infantis cluster. However, their ribosomal proteins have both characters of longum and infantis types. This result suggests that the taxonomic position of the subsp. suis should be reconsidered.
巻・号 34(1)
ページ 76-80
公開日 2011-2-1
DOI 10.1016/j.syapm.2010.07.003
PII S0723-2020(10)00098-6
PMID 20828962
MeSH Bacterial Proteins / analysis* Bacteriological Techniques / methods* Bifidobacterium / chemistry* Bifidobacterium / classification* Cluster Analysis Phylogeny Ribosomal Proteins / analysis* Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / methods*
IF 3.224
引用数 24
WOS 分野 BIOTECHNOLOGY & APPLIED MICROBIOLOGY MICROBIOLOGY
リソース情報
一般微生物 JCM 1210 JCM 1222 JCM 1260 JCM 1272 JCM 7007 JCM 7009 JCM 7010 JCM 7011 JCM 1217 JCM 7050 JCM 7052 JCM 7053 JCM 7054 JCM 7055 JCM 7056 JCM 1269 JCM 7139