Reference - Detail
RRC ID | 47570 |
---|---|
Author | Teramoto H, Inui M, Yukawa H. |
Title | Corynebacterium glutamicum CsoR acts as a transcriptional repressor of two copper/zinc-inducible P(1B)-type ATPase operons. |
Journal | Biosci Biotechnol Biochem |
Abstract |
The mechanism of regulation of the expression of copA and copB, encoding putative copper-translocating P(1B)-type ATPases in Corynebacterium glutamicum, was investigated. The levels of copA and copB mRNAs were upregulated in response to excess copper as well as excess zinc. Disruption of csoR, encoding a transcriptional regulator, resulted in constitutive expression of copA and copB. The CsoR protein bound to the promoter regions of the copA-csoR and the cgR_0124-copB-cgR_0126 operon. In vitro DNA binding activity was strongly inhibited by copper, but much less inhibited by zinc. A csoR-deficient mutant showed slightly increased resistance to copper, but slightly decreased resistance to zinc. These findings indicate that CsoR acts as a transcriptional repressor not only of the cognate copA-csoR operon but also of the cgR_0124-copB-cgR_0126 operon, which is not physically linked to csoR on the chromosome, and that CsoR plays a major role in copper homeostasis. |
Volume | 76(10) |
Pages | 1952-8 |
Published | 2012-1-1 |
DOI | 10.1271/bbb.120437 |
PII | DN/JST.JSTAGE/bbb/120437 |
PMID | 23090582 |
MeSH | Adenosine Triphosphatases / genetics* Adenosine Triphosphatases / metabolism Bacterial Proteins / metabolism* Base Sequence Biological Transport Copper / metabolism Copper / pharmacology* Corynebacterium glutamicum / enzymology Corynebacterium glutamicum / genetics Corynebacterium glutamicum / metabolism* DNA, Bacterial / metabolism Gene Expression Regulation, Bacterial / drug effects Gene Expression Regulation, Bacterial / genetics Genome, Bacterial / drug effects Genome, Bacterial / genetics Operon / drug effects* Operon / genetics Promoter Regions, Genetic / drug effects Promoter Regions, Genetic / genetics Repressor Proteins / metabolism* Zinc / pharmacology* |
IF | 1.516 |
Times Cited | 7 |
WOS Category | FOOD SCIENCE & TECHNOLOGY BIOTECHNOLOGY & APPLIED MICROBIOLOGY CHEMISTRY, APPLIED BIOCHEMISTRY & MOLECULAR BIOLOGY |
Resource | |
General Microbes | JCM 18229 |