RRC ID 47579
Author Iwabuchi N, Yonezawa S, Odamaki T, Yaeshima T, Iwatsuki K, Xiao JZ.
Title Immunomodulating and anti-infective effects of a novel strain of Lactobacillus paracasei that strongly induces interleukin-12.
Journal FEMS Immunol. Med. Microbiol.
Abstract Several studies have demonstrated that some strains of lactic acid bacteria (LAB) can elicit natural killer (NK) cell activities via interleukin-12 (IL-12) induction and protect against influenza virus (IFV) infection. LAB strains that strongly induce IL-12 are expected to be effective in protecting against IFV infection. In this study, we screened 85 strains for their ability to induce the in vitro production of IL-12, and Lactobacillus paracasei MoLac-1 most strongly induced IL-12. To examine the immunomodulating effects of MoLac-1, we have performed in vitro studies using murine splenocytes. Heat-killed MoLac-1 cells induced IL-12 and interferon-γ (IFN-γ) production by murine splenocytes. Experiments using splenocytes depleted of various cell populations indicated that macrophages might be a major source of MoLac-1-induced IL-12 secretion. Intracellular staining of IFN-γ suggested that MoLac-1 activated NK cells and induced IFN-γ production by NK cells in vitro. Oral administration of heat-killed MoLac-1 increased the proportion of NK cells in spleen, and ameliorated the symptoms of IFV infection in mice. These results suggest that heat-killed MoLac-1 has the potential to modulate innate immunity and is useful for alleviation of the symptoms of IFV infection.
Volume 66(2)
Pages 230-9
Published 2012-11
DOI 10.1111/j.1574-695X.2012.01003.x
PMID 22775119
MeSH Animals Disease Models, Animal Interferon-gamma / secretion Interleukin-12 / secretion* Killer Cells, Natural / immunology Killer Cells, Natural / microbiology Lactobacillus / immunology* Leukocytes, Mononuclear / immunology* Leukocytes, Mononuclear / microbiology* Macrophages / immunology Macrophages / microbiology Mice Mice, Inbred BALB C Orthomyxoviridae / immunology Orthomyxoviridae Infections / pathology Orthomyxoviridae Infections / therapy Spleen / immunology
Times Cited 6
WOS Category INFECTIOUS DISEASES MICROBIOLOGY IMMUNOLOGY
Resource
General Microbes JCM 8713