RRC ID |
47579
|
Author |
Iwabuchi N, Yonezawa S, Odamaki T, Yaeshima T, Iwatsuki K, Xiao JZ.
|
Title |
Immunomodulating and anti-infective effects of a novel strain of Lactobacillus paracasei that strongly induces interleukin-12.
|
Journal |
FEMS Immunol Med Microbiol
|
Abstract |
Several studies have demonstrated that some strains of lactic acid bacteria (LAB) can elicit natural killer (NK) cell activities via interleukin-12 (IL-12) induction and protect against influenza virus (IFV) infection. LAB strains that strongly induce IL-12 are expected to be effective in protecting against IFV infection. In this study, we screened 85 strains for their ability to induce the in vitro production of IL-12, and Lactobacillus paracasei MoLac-1 most strongly induced IL-12. To examine the immunomodulating effects of MoLac-1, we have performed in vitro studies using murine splenocytes. Heat-killed MoLac-1 cells induced IL-12 and interferon-γ (IFN-γ) production by murine splenocytes. Experiments using splenocytes depleted of various cell populations indicated that macrophages might be a major source of MoLac-1-induced IL-12 secretion. Intracellular staining of IFN-γ suggested that MoLac-1 activated NK cells and induced IFN-γ production by NK cells in vitro. Oral administration of heat-killed MoLac-1 increased the proportion of NK cells in spleen, and ameliorated the symptoms of IFV infection in mice. These results suggest that heat-killed MoLac-1 has the potential to modulate innate immunity and is useful for alleviation of the symptoms of IFV infection.
|
Volume |
66(2)
|
Pages |
230-9
|
Published |
2012-11-1
|
DOI |
10.1111/j.1574-695X.2012.01003.x
|
PMID |
22775119
|
MeSH |
Animals
Disease Models, Animal
Interferon-gamma / metabolism
Interleukin-12 / metabolism*
Killer Cells, Natural / immunology
Killer Cells, Natural / microbiology
Lactobacillus / immunology*
Leukocytes, Mononuclear / immunology*
Leukocytes, Mononuclear / microbiology*
Macrophages / immunology
Macrophages / microbiology
Mice
Mice, Inbred BALB C
Orthomyxoviridae / immunology
Orthomyxoviridae Infections / pathology
Orthomyxoviridae Infections / therapy
Spleen / immunology
|
Times Cited |
11
|
WOS Category
|
INFECTIOUS DISEASES
MICROBIOLOGY
IMMUNOLOGY
|
Resource |
General Microbes |
JCM 8713 |