RRC ID 47596
著者 Matsuda N, Matsuda M, Notake S, Yokokawa H, Kawamura Y, Hiramatsu K, Kikuchi K.
タイトル Evaluation of a simple protein extraction method for species identification of clinically relevant staphylococci by matrix-assisted laser desorption ionization-time of flight mass spectrometry.
ジャーナル J Clin Microbiol
Abstract In clinical microbiology, bacterial identification is labor-intensive and time-consuming. A solution for this problem is the use of matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS). In this study, we evaluated a modified protein extraction method of identification performed on target plates (on-plate extraction method) with MALDI-TOF (Bruker Microflex LT with Biotyper version 3.0) and compared it to 2 previously described methods: the direct colony method and a standard protein extraction method (standard extraction method). We evaluated the species of 273 clinical strains and 14 reference strains of staphylococci. All isolates were characterized using the superoxide dismutase A sequence as a reference. For the species identification, the on-plate, standard extraction, and direct colony methods identified 257 isolates (89.5%), 232 isolates (80.8%), and 173 isolates (60.2%), respectively, with statistically significant differences among the three methods (P < 0.05). In conclusion, the on-plate extraction method is at least as good as standard extraction in identification rate and has the advantage of a shorter processing time.
巻・号 50(12)
ページ 3862-6
公開日 2012-12-1
DOI 10.1128/JCM.01512-12
PII JCM.01512-12
PMID 22993187
PMC PMC3502947
MeSH Bacterial Proteins / analysis* Bacterial Proteins / isolation & purification* Bacteriological Techniques / methods Humans Specimen Handling / methods* Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / methods* Staphylococcal Infections / diagnosis Staphylococcal Infections / microbiology Staphylococcus / chemistry* Staphylococcus / classification* Staphylococcus / isolation & purification
IF 5.897
引用数 28
WOS 分野 MICROBIOLOGY
リソース情報
一般微生物 JCM 2427 JCM 7470