論文 - 詳細
| RRC ID | 47596 |
|---|---|
| 著者 | Matsuda N, Matsuda M, Notake S, Yokokawa H, Kawamura Y, Hiramatsu K, Kikuchi K. |
| タイトル | Evaluation of a simple protein extraction method for species identification of clinically relevant staphylococci by matrix-assisted laser desorption ionization-time of flight mass spectrometry. |
| ジャーナル | J Clin Microbiol |
| Abstract |
In clinical microbiology, bacterial identification is labor-intensive and time-consuming. A solution for this problem is the use of matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS). In this study, we evaluated a modified protein extraction method of identification performed on target plates (on-plate extraction method) with MALDI-TOF (Bruker Microflex LT with Biotyper version 3.0) and compared it to 2 previously described methods: the direct colony method and a standard protein extraction method (standard extraction method). We evaluated the species of 273 clinical strains and 14 reference strains of staphylococci. All isolates were characterized using the superoxide dismutase A sequence as a reference. For the species identification, the on-plate, standard extraction, and direct colony methods identified 257 isolates (89.5%), 232 isolates (80.8%), and 173 isolates (60.2%), respectively, with statistically significant differences among the three methods (P < 0.05). In conclusion, the on-plate extraction method is at least as good as standard extraction in identification rate and has the advantage of a shorter processing time. |
| 巻・号 | 50(12) |
| ページ | 3862-6 |
| 公開日 | 2012-12-1 |
| DOI | 10.1128/JCM.01512-12 |
| PII | JCM.01512-12 |
| PMID | 22993187 |
| PMC | PMC3502947 |
| MeSH | Bacterial Proteins / analysis* Bacterial Proteins / isolation & purification* Bacteriological Techniques / methods Humans Specimen Handling / methods* Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / methods* Staphylococcal Infections / diagnosis Staphylococcal Infections / microbiology Staphylococcus / chemistry* Staphylococcus / classification* Staphylococcus / isolation & purification |
| IF | 5.897 |
| 引用数 | 28 |
| WOS 分野 | MICROBIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 3 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 一般微生物 | JCM 2427 JCM 7470 |