RRC ID 4760
Author Shirota Y, Kaneko S, Honda M, Kawai HF, Kobayashi K.
Title Identification of differentially expressed genes in hepatocellular carcinoma with cDNA microarrays.
Journal Hepatology
Abstract Genes expressed in hepatocellular carcinoma (HCC) were analyzed using cDNA microarrays to clarify gene abnormalities in HCC. mRNA was extracted from cancerous and noncancerous tissues of 10 patients with HCC, cDNA labeled with Cy5 and Cy3 fluorescence was prepared, and it was hybridized for each patient with a cDNA microarray consisting of 1,080 elements (930 unique genes). The mRNA expression rate of each element in HCC was evaluated using the level of mRNA expression in noncancerous tissue in each patient as a reference. The expression of 10 genes was enhanced 2 times or more in HCC cancerous tissue compared with noncancerous tissue in 5 or more of the 10 patients. In contrast, 9 genes were expressed at half the level or less in HCC cancerous tissue compared with noncancerous tissue. When hierarchical clustering was performed to identify genes related to clinical phenotypes of the patients, 22 genes showed changes associated with the degree of differentiation of HCC. Thirteen of these genes were transcriptional factors or tissue-specific expression proteins related to cell differentiation or development. Our present analysis clarified a number of genes that characterize HCC. This information based on examination of clinical samples is considered to be useful for clarification of the mechanism of hepatocarcinogenesis and the diagnosis and treatment of HCC.
Volume 33(4)
Pages 832-40
Published 2001-4-1
DOI 10.1053/jhep.2001.23003
PII S0270-9139(01)23292-3
PMID 11283847
MeSH Adult Aged Carcinoma, Hepatocellular / genetics* Carcinoma, Hepatocellular / pathology DNA, Complementary / genetics* Female Gene Expression Profiling* Humans Liver / physiology Liver Neoplasms / genetics* Liver Neoplasms / pathology Male Middle Aged Oligonucleotide Array Sequence Analysis* Reference Values
IF 14.679
Times Cited 150
DNA material Transforming growth factor beta receptor type IIB (RDB01606)