| RRC ID |
47600
|
| 著者 |
Sakata J, Kawatsu K, Iwasaki T, Tanaka K, Takenaka S, Kumeda Y, Kodama H.
|
| タイトル |
Production and characterization of a novel monoclonal antibody against Vibrio parahaemolyticus F0F1 ATP synthase's delta subunit and its application for rapid identification of the pathogen.
|
| ジャーナル |
J Microbiol Methods
|
| Abstract |
We raised monoclonal antibodies (MAbs) against Vibrio parahaemolyticus cell extracts. One of the MAbs, designated MAb-VP34, reacted in enzyme-linked immunosorbent assays (ELISAs) with 140 V. parahaemolyticus strains, regardless of serotype or origin. MAb-VP34 did not detectably react with 96 strains belonging to 27 other Vibrio species (except for Vibrio natriegens) or with 29 non-Vibrio species. These results show that MAb-VP34 is highly specific for V. parahaemolyticus. Western blotting and mass spectrometry analyses revealed that MAb-VP34 recognized V. parahaemolyticus F(0)F(1) ATP synthase's delta subunit. Using MAb-VP34, a rapid and simple immunodot blotting assay (VP-Dot) was developed to determine whether bacterial colonies growing on selective agar, represented V. parahaemolyticus. To evaluate VP-Dot, 20 V. parahaemolyticus strains and 19 non-related strains were tested. The results indicated that VP-Dot is a reliable tool for identification of V. parahaemolyticus colonies. The simple VP-Dot procedure took 40min, indicating that the MAb-VP34 based immunological method will greatly reduce labor, time, and costs required to verify V. parahaemolyticus colonies as compared with the conventional biochemical test.
|
| 巻・号 |
88(1)
|
| ページ |
77-82
|
| 公開日 |
2012-1-1
|
| DOI |
10.1016/j.mimet.2011.10.014
|
| PII |
S0167-7012(11)00368-X
|
| PMID |
22056232
|
| MeSH |
Animals
Antibodies, Monoclonal / analysis*
Bacterial Proteins / immunology*
Bacterial Typing Techniques / instrumentation
Bacterial Typing Techniques / methods*
Female
Humans
Immunoblotting / instrumentation
Immunoblotting / methods*
Mice
Mice, Inbred BALB C
Protein Subunits / immunology
Proton-Translocating ATPases / immunology*
Seafood / microbiology
Vibrio Infections / microbiology*
Vibrio parahaemolyticus / classification
Vibrio parahaemolyticus / enzymology
Vibrio parahaemolyticus / immunology
Vibrio parahaemolyticus / isolation & purification*
|
| IF |
1.707
|
| 引用数 |
8
|
|
WOS 分野
|
BIOCHEMICAL RESEARCH METHODS
MICROBIOLOGY
|
| リソース情報 |
| 一般微生物 |
JCM 1235
JCM 1665
JCM 1663
JCM 1672 |
