| RRC ID |
47601
|
| 著者 |
Tsukatani T, Suenaga H, Shiga M, Noguchi K, Ishiyama M, Ezoe T, Matsumoto K.
|
| タイトル |
Comparison of the WST-8 colorimetric method and the CLSI broth microdilution method for susceptibility testing against drug-resistant bacteria.
|
| ジャーナル |
J Microbiol Methods
|
| Abstract |
The minimum inhibitory concentrations (MICs) obtained from the susceptibility testing of various bacteria to antibiotics were determined by a colorimetric microbial viability assay based on reduction of a tetrazolium salt {2-(2-methoxy-4-nitrophenyl)-3-(4-nitrophenyl)-5-(2,4-disulfophenyl)-2H-tetrazolium, monosodium salt (WST-8)} via 2-methyl-1,4-napthoquinone as an electron mediator and compared with those obtained by the broth microdilution methods approved by the Clinical and Laboratory Standard Institute (CLSI). Especially for drug-resistant bacteria, the CLSI method at an incubation time of 24h tended to give lower MICs. The extension of incubation time was necessary to obtain consistent MICs for drug-resistant bacteria such as methicillin-resistant Staphylococcus aureus (MRSA), vancomycin-resistant enterococi (VRE) and multi-drug resistant Pseudomonas aeruginosa (MDRP) in the broth microdilution method. There was excellent agreement between the MICs determined after 24h using the WST-8 colorimetric method and those obtained after 48-96 h using the broth microdilution method. The results suggest that the WST-8 colorimetric assay is a useful method for rapid determination of consistent MICs for drug-resistant bacteria.
|
| 巻・号 |
90(3)
|
| ページ |
160-6
|
| 公開日 |
2012-9-1
|
| DOI |
10.1016/j.mimet.2012.05.001
|
| PII |
S0167-7012(12)00168-6
|
| PMID |
22642794
|
| MeSH |
Anti-Bacterial Agents / pharmacology*
Ciprofloxacin / pharmacology
Colorimetry
Culture Media
Enterococcus faecalis / drug effects
Enterococcus faecalis / growth & development
Haemophilus influenzae / drug effects
Haemophilus influenzae / growth & development
Indicator Dilution Techniques
Klebsiella pneumoniae / drug effects
Klebsiella pneumoniae / growth & development
Methicillin-Resistant Staphylococcus aureus / drug effects
Methicillin-Resistant Staphylococcus aureus / growth & development
Microbial Sensitivity Tests / methods*
Microbial Viability / drug effects
Pseudomonas aeruginosa / drug effects
Pseudomonas aeruginosa / growth & development
Vancomycin / pharmacology
Vancomycin Resistance*
beta-Lactam Resistance*
beta-Lactams / pharmacology
|
| IF |
1.707
|
| 引用数 |
97
|
|
WOS 分野
|
BIOCHEMICAL RESEARCH METHODS
MICROBIOLOGY
|
| リソース情報 |
| 一般微生物 |
JCM 8702
JCM 5803
JCM 6841 |
