| RRC ID |
47608
|
| 著者 |
Osaki T, Matsuki T, Asahara T, Zaman C, Hanawa T, Yonezawa H, Kurata S, Woo TD, Nomoto K, Kamiya S.
|
| タイトル |
Comparative analysis of gastric bacterial microbiota in Mongolian gerbils after long-term infection with Helicobacter pylori.
|
| ジャーナル |
Microb Pathog
|
| Abstract |
Quantitative (qt) real time PCR using 16SrDNA primers is useful for determination of the bacterial composition of the gastric microbiota in Mongolian gerbils. The aim of this study was to determine the change in the gastric microbiota after long-term infection with Helicobacter pylori. One year after inoculation with H. pylori, five gerbils were determined as H. pylori-positive and 6 gerbils H. pylori-negative by culture and real time qt PCR methods. The gastric microbiota of each group of gerbils was also compared with that of 6 gerbils uninfected with H. pylori. DNA from the Atopobium cluster, Bifidobacterium spp., Clostridium coccoides group, Clostridium leptum subgroup, Enterococcus spp. and Lactobacillus spp. were detected in the gastric mucus of both infected and uninfected gerbils. In contrast, Eubacterium cylindroides group and Prevotella spp. were detected only in H. pylori-negative gerbils. The numbers of C. leptum subgroup, C. coccoides group and Bifidobacterium spp. in gastric mucus of H. pylori-negative Mongolian gerbils were significantly lower than those in non-infected gerbils. The results obtained suggest that the composition of gastric indigenous microbiota in Mongolian gerbils may be disturbed by long-term infection with H. pylori, and that these changes may in fact inhibit H. pylori infection.
|
| 巻・号 |
53(1)
|
| ページ |
12-8
|
| 公開日 |
2012-7-1
|
| DOI |
10.1016/j.micpath.2012.03.008
|
| PMID |
22783557
|
| MeSH |
Animals
Biodiversity*
DNA Primers / genetics
Disease Models, Animal
Gastric Juice / microbiology
Gerbillinae / microbiology*
Helicobacter Infections / microbiology*
Helicobacter pylori / growth & development*
Male
Mucus / microbiology
RNA, Ribosomal, 16S / genetics
Real-Time Polymerase Chain Reaction
Stomach / microbiology*
|
| IF |
2.914
|
| 引用数 |
33
|
|
WOS 分野
|
MICROBIOLOGY
IMMUNOLOGY
|
| リソース情報 |
| 一般微生物 |
JCM 1134
JCM 5803 |
