| RRC ID |
47613
|
| Author |
Pedrolli DB, Matern A, Wang J, Ester M, Siedler K, Breaker R, Mack M.
|
| Title |
A highly specialized flavin mononucleotide riboswitch responds differently to similar ligands and confers roseoflavin resistance to Streptomyces davawensis.
|
| Journal |
Nucleic Acids Res
|
| Abstract |
Streptomyces davawensis is the only organism known to synthesize the antibiotic roseoflavin, a riboflavin (vitamin B2) analog. Roseoflavin is converted to roseoflavin mononucleotide (RoFMN) and roseoflavin adenine dinucleotide in the cytoplasm of target cells. (Ribo-)Flavin mononucleotide (FMN) riboswitches are genetic elements, which in many bacteria control genes responsible for the biosynthesis and transport of riboflavin. Streptomyces davawensis is roseoflavin resistant, and the closely related bacterium Streptomyces coelicolor is roseoflavin sensitive. The two bacteria served as models to investigate roseoflavin resistance of S. davawensis and to analyze the mode of action of roseoflavin in S. coelicolor. Our experiments demonstrate that the ribB FMN riboswitch of S. davawensis (in contrast to the corresponding riboswitch of S. coelicolor) is able to discriminate between the two very similar flavins FMN and RoFMN and shows opposite responses to the latter ligands.
|
| Volume |
40(17)
|
| Pages |
8662-73
|
| Published |
2012-9-1
|
| DOI |
10.1093/nar/gks616
|
| PII |
gks616
|
| PMID |
22740651
|
| PMC |
PMC3458559
|
| MeSH |
Anti-Bacterial Agents / pharmacology*
Aptamers, Nucleotide / metabolism
Cytoplasm / metabolism
Drug Resistance, Fungal
Flavin Mononucleotide / metabolism
Flavin-Adenine Dinucleotide / analogs & derivatives
Flavin-Adenine Dinucleotide / metabolism
Gene Expression Regulation, Fungal*
Genome, Fungal
Ligands
Point Mutation
Protein Biosynthesis
Riboflavin / analogs & derivatives
Riboflavin / metabolism
Riboflavin / pharmacology
Riboflavin Synthase / metabolism
Riboswitch*
Streptomyces / drug effects
Streptomyces / enzymology
Streptomyces / genetics*
Streptomyces coelicolor / drug effects
Streptomyces coelicolor / enzymology
Streptomyces coelicolor / genetics
|
| IF |
11.502
|
| Times Cited |
38
|
|
WOS Category
|
BIOCHEMISTRY & MOLECULAR BIOLOGY
|
| Resource |
| General Microbes |
JCM 4913 |
